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1. Introduction
2. Anterior wall myocardial infraction
3. Establish early reperfusion
4. Reperfusion injury
5. Mechanisms of reperfusion injury
6. Oxygen radicals are central mediators of injury
7. Free radicals in biology
8. Evidence for the role of oxygen radicals in injury
9. Pressure recovery after ischemia and reperfusion
10. Treatment with hSOD in vivo
11. Electron paramagnetic/spin resonance
12. 5,5-dimethyl-1-pyrroline N-oxide (DMPO)
13. EPR spin trapping experiment
14. Ischemic duration with maximum radical formation
15. Radical generation depends on ischemic duration
16. Treatment with active and inactive SOD
17. SOD1 over-expression prevents reperfusion injury
18. Reoxygenated endothelial cells generate radicals
19. Xanthine oxidase - a source of radical generation
20. XO and XDH are present in the heart
21. XO substrates are formed during ischemia
22. EPR spin trapping of endothelial cells and hearts
23. Superoxide and functional adhesion
24. Superoxide affects adhesion molecule expression
25. Superoxide activates leukocyte adhesion
26. Role of neutrophils and plasma factors in injury
27. Leukocytes and complement enhance injury
28. Leukocytes, complement and radical generation
29. Leukocytes, complement and injury - conclusions
30. Cellular mechanisms of radical generation
31. Important biological radicals
32. The reaction catalyzed by NO synthase (NOS)
33. NOS activity in L-arginine-depleted cells
34. N-methyl-D-glucamine dithiocarbamate (MGD)
35. EPR spectra of nitric oxide in MGD labeled hearts
36. Time course of NO generation during ischemia
37. NO generation in the ischemic heart
38. Imaging of nitric oxide in the rat heart
39. NO-heme complexes formation in ischemic heart
40. Myocardial salvage in acute myocardial infarction
41. How can we translate this to the clinic?
42. Low molecular weight SOD-mimetic (M40403)
43. Methods
44. Progression of infarct size over time
45. Adjusted mean values for infract size
46. SOD-mimetic (M40403) study - conclusions
47. EPR imaging system setup
48. EPR imaging
49. EPR imaging of oxygen
50. EPR imaging of redox state
51. Conclusions
52. Acknowledgments
53. The OSU heart center

Topics Covered

• Reperfusion injury
• Oxygen radicals are mediators of injury
• Reperfusion radical generation and duration of ischemia
• SOD1 and reperfusion
• Xanthine oxidase as a source of radical generation
• Xanthine oxidase substrates are formed during ischemia
• Superoxide and adhesion molecule expression
• Post-ischemic injury and leukocytes
• NO generation during ischemia
• NO-heme complexes in ischemic heart
• Maximizing myocardial salvage
• EPR imaging

Links

Series:

• Free Radicals and the Oxygen Paradox

Categories:

• Biochemistry
• Cell Biology
• Clinical Practice

Therapeutic Areas:

• Cardiovascular & Metabolic

Talk Citation

Zweier, J. (2007, November 1). Oxidative stress in disease: cardiac ischemia/reperfusion injury [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/BRTZ6744.
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Publication History

• Published on November 1, 2007
• Archived on May 31, 2018

Financial Disclosures

• Prof. Jay Zweier has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.