• Introduction and General Properties of Plasmids
• 31 min
  1. Introduction to plasmid biology

  • Prof. Emeritus Don Clewell
• 39 min
  2. General properties and applications of plasmids

  • Prof. Emeritus Don Clewell
• Replication
• 47 min
  3. Plasmids: copy number control by antisense RNAs

  • Prof. Gerhart Wagner
• 30 min
  4. Plasmid rolling-circle (RC) replication

  • Prof. Saleem Khan
• 45 min
  5. Replication of linear plasmids in bacteria

  • Prof. George Chaconas
• Maintenance and Partitioning
• 32 min
  6. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 33 min
  7. Plasmid stabilization by cell killing

  • Prof. Keith Weaver
• 30 min
  8. Dimer catastrophes and plasmid resolution

  • Dr. David Summers
• Conjugation and Mobilization
• 41 min
  9. The diversity of bacterial conjugation and mobilization systems

  • Prof. Fernando de la Cruz
• 36 min
  10. The pCF10-encoded pheromone response: cell-cell signaling, complexity and evolution

  • Prof. Gary Dunny
• 27 min
  11. DNA processing in conjugative transfer

  • Dr. Joel Schildbach
• 43 min
  12. Agrobacterium tumefaciens

  • Dr. Stephen Winans
• Genetic Traits Frequently Associated with Plasmids
• 37 min
  13. Insertion sequences and DNA transposition

  • Prof. William Reznikoff
• 48 min
  14. Plasmids, integrons and the spread of antibiotic resistance

  • Prof. Hatch Stokes
• 30 min
  15. Virulence plasmids

  • Prof. Julian Rood
• Eukaryotic Plasmids
• 30 min
  16. The yeast plasmid: a highly persistent selfish DNA element

  • Prof. Makkuni Jayaram
• Population Biology and Evolution
• 35 min
  17. Evolution of plasmids and their role in bacterial diversity and adaptability

  • Prof. Christopher Thomas
• Archived Lectures *These may not cover the latest advances in the field
• 85 min
  18. Bacterial extrachromosomal elements: role in chemical detoxification and potential cancer therapy

  • Prof. Ananda Chakrabarty
• 34 min
  19. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 39 min
  20. Replication of linear plasmids in bacteria

  • Prof. George Chaconas

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Lateral gene transfer
3. The integron/gene cassette system
4. Arrangement of integron/gene cassette system
5. The recombination system
6. Three components of an integron
7. Gene cassettes
8. Circular form of genes cassettes
9. Linear form of a gene cassette
10. Gene cassette capture
11. Recombination occures in two directions
12. What integrons can do
13. Cassette associated genes
14. Integrons and multi drug resistance
15. Example: resistance genes in class 1 integrons
16. Examples of class 1 integron arrays
17. Cassettes can appear in different contexts
18. The cassette recombination site
19. Cassette recombination site structure
20. 59-be sites are imperfect inverted repeats
21. Examples of 59-be sites
22. A typical cassette
23. Elements in linear cassettes are hybrids
24. Integron/gene cassette biology
25. Integrons in pathogens
26. Integron classes (1)
27. Integron classes (2)
28. Distribution of chromosomal integrons
29. Chromosomal integrons have large arrays
30. Cassette genes are novel (1)
31. Cassette genes are novel (2)
32. Reminder: integron/gene cassette biology
33. How have class 1 integrons been spread?
34. Functional features of class 1 integrons
35. Class 1 integrons have additional features
36. Two definitions of class 1 integrons
37. Physical structure of class 1 integrons
38. Evolution of class 1 structure
39. Ancestral structure: Tn402
40. Tn402 - a transposon and a functional integron
41. Inverted repeats allow transposition of integrons
42. Spread of class 1 integrons
43. A transposon in transposons
44. Class 1 integrons in the Tn3 family
45. Mobile elements interact
46. Consquence of spreading resistance genes
47. Other mobilized integron classes exist
48. References

Topics Covered

• A description of the integron/gene cassette system
• Integrons in the general environment
• Class 1 integron mediated spread of antibiotic resistance genes in pathogens
• How class 1 integrons work with plasmids and transposons to spread between cells

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on January 28, 2008

Financial Disclosures

• Prof. Hatch Stokes has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.