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1. Introduction
2. Talk outline
3. What are IS elements?
4. Why are IS elements interesting?
5. Discovery of insertion sequences (IS elements)
6. Transposition
7. IS structure
8. Gamma-delta and Tn4
9. IS elements can form composite transposons
10. Composite transposon formation
11. Cut and paste vs. replicative transposition
12. Mechanism of cut and paste IS transposition
13. IS50-like transposition
14. Crystal structure of the IS50 synaptic complex
15. Transposase proteins
16. Regulation of IS transposition
17. Role of IS elements in bacterial genetics
18. Schematic of the F plasmid
19. IS-mediated integration of F to generate HFR
20. IS-mediated F' formation
21. F' formation by deletion formation
22. R factors
23. Conclusion

Topics Covered

• Insertions sequences
• A major form of mobile genetic elements in eubacteria and archaebacteria
• Insertion sequences move via DNA transposition
• Insertion sequences cause genome changes (insertions, deletions, inversions and chromosome fusions) and facilitate the horizontal transfer of genes
• Related processes include integration of HIV-1 DNA

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Talk Citation

Publication History

• Published on January 28, 2008
• Archived on May 28, 2025

Financial Disclosures

• Prof. William Reznikoff has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.