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1. Introduction
2. Plasmids
3. Conjugative plasmids
4. Lederberg and Tatum experiments
5. Bacterial conjugation
6. Conjugative transfer requirements
7. Processes required for transfer
8. Example: F factor
9. DNA processing in F conjugative transfer
10. Transfer genes on F factor
11. F plasmid oriT
12. F plasmid tra operon
13. TraY and IHF
14. F plasmid oriT and TraI
15. Model for F factor transfer initiation
16. Molecular mechanisms and regulation
17. F oriT near nic
18. DNA form pre- and post-cleavage
19. Evidence for context-dependent TraI recognition
20. Other questions
21. References

Topics Covered

• Conjugative plasmids
• Original observation of conjugative transfer
• Description of transfer
• DNA specificity in conjugative transfer
• F transfer as an example
• Roles of F proteins in transfer
• Context dependence of F TraI recognition as regulation

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Talk Citation

Publication History

• Published on January 28, 2008

Financial Disclosures

• Dr. Joel Schildbach has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.