• Introduction and General Properties of Plasmids
• 31 min
  1. Introduction to plasmid biology

  • Prof. Emeritus Don Clewell
• 39 min
  2. General properties and applications of plasmids

  • Prof. Emeritus Don Clewell
• Replication
• 47 min
  3. Plasmids: copy number control by antisense RNAs

  • Prof. Gerhart Wagner
• 30 min
  4. Plasmid rolling-circle (RC) replication

  • Prof. Saleem Khan
• 45 min
  5. Replication of linear plasmids in bacteria

  • Prof. George Chaconas
• Maintenance and Partitioning
• 32 min
  6. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 33 min
  7. Plasmid stabilization by cell killing

  • Prof. Keith Weaver
• 30 min
  8. Dimer catastrophes and plasmid resolution

  • Dr. David Summers
• Conjugation and Mobilization
• 41 min
  9. The diversity of bacterial conjugation and mobilization systems

  • Prof. Fernando de la Cruz
• 36 min
  10. The pCF10-encoded pheromone response: cell-cell signaling, complexity and evolution

  • Prof. Gary Dunny
• 27 min
  11. DNA processing in conjugative transfer

  • Dr. Joel Schildbach
• 43 min
  12. Agrobacterium tumefaciens

  • Dr. Stephen Winans
• Genetic Traits Frequently Associated with Plasmids
• 37 min
  13. Insertion sequences and DNA transposition

  • Prof. William Reznikoff
• 48 min
  14. Plasmids, integrons and the spread of antibiotic resistance

  • Prof. Hatch Stokes
• 30 min
  15. Virulence plasmids

  • Prof. Julian Rood
• Eukaryotic Plasmids
• 30 min
  16. The yeast plasmid: a highly persistent selfish DNA element

  • Prof. Makkuni Jayaram
• Population Biology and Evolution
• 35 min
  17. Evolution of plasmids and their role in bacterial diversity and adaptability

  • Prof. Christopher Thomas
• Archived Lectures *These may not cover the latest advances in the field
• 85 min
  18. Bacterial extrachromosomal elements: role in chemical detoxification and potential cancer therapy

  • Prof. Ananda Chakrabarty
• 34 min
  19. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 39 min
  20. Replication of linear plasmids in bacteria

  • Prof. George Chaconas

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Outline
3. Transfer of DNA between bacterial cells
4. Basic plasmid platforms
5. Tra vs. Mob: R388 and RSF1010
6. The mechanism of conjugation
7. The key components in conjugation
8. TrwB - a coupling protein
9. Related DNA transport processes
10. The mating channel
11. Liquid vs. surface maters
12. The relaxosome
13. Shoot and pump conjugation model
14. The complex between TrwC and its substrate DNA
15. Catalytic center mutagenesis
16. TrwC relaxase protein family
17. Conserved relaxase motifs in six relaxase families
18. 3D relaxase structures
19. Diversity and classification of conjugation systems
20. The IncW family
21. IncW backbone
22. Segregational incompatibility
23. Methods for plasmid classification
24. Well known plasmid Inc groups in Enterobacteria
25. Conserved relaxase motifs in six relaxase families
26. BLAST searches against prototype relaxases
27. MOB-P relaxase family
28. The MOB-F family
29. MOB-H relaxase family
30. MOB-C family
31. Classification by relaxases vs. T4CP and T4SS
32. Plasmid MOB families
33. MOB families distribution and relaxase signatures
34. A search on the NCBI plasmid database
35. Progression in plasmid sequencing
36. Plasmid dstribution in bacterial host phyla
37. Plasmid distribution according to size
38. Conjugative vs. mobilizable plasmids
39. Relaxase families in 1,010 plasmids
40. MOBs distribution according to plasmid size
41. Relaxases distribution in main bacterial phyla
42. Relaxases dstribution in phylum Proteobacteria
43. NCBI plasmid database analysis: conclusions
44. Acknowledgements

Topics Covered

• Transfer of DNA between bacterial cells
• Basic plasmid platforms
• The mechanism and key components of conjugation
• The mating channel
• The complex between TrwC and its substrate DNA
• The relaxosome
• The diversity of relaxases
• The IncW family
• Methods for plasmid classification
• Plasmid MOB families
• The NCBI plasmid database
• Plasmid sequencing

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Talk Citation

Publication History

• Published on January 28, 2008

Financial Disclosures

• Prof. Fernando de la Cruz has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.