We noted you are experiencing viewing problems
-
Check with your IT department that JWPlatform, JWPlayer and Amazon AWS & CloudFront are not being blocked by your network. The relevant domains are *.jwplatform.com, *.jwpsrv.com, *.jwpcdn.com, jwpltx.com, jwpsrv.a.ssl.fastly.net, *.amazonaws.com and *.cloudfront.net. The relevant ports are 80 and 443.
-
Check the following talk links to see which ones work correctly:
Auto Mode
HTTP Progressive Download Send us your results from the above test links at access@hstalks.com and we will contact you with further advice on troubleshooting your viewing problems. -
No luck yet? More tips for troubleshooting viewing issues
-
Contact HST Support access@hstalks.com
-
Please review our troubleshooting guide for tips and advice on resolving your viewing problems.
-
For additional help, please don't hesitate to contact HST support access@hstalks.com
We hope you have enjoyed this limited-length demo
This is a limited length demo talk; you may
login or
review methods of
obtaining more access.
Printable Handouts
Navigable Slide Index
- Introduction
- Alternative oxidative metabolism enzymes
- Lecture summary
- Molybdenum hydroxylases
- Properties of molybdenum hydroxylases
- Reactions catalysed by AO and XO
- Substrate specificity of AO and XO
- Molybdenum hydroxylase catalysed oxidation
- In vivo electron transfer xanthine dehydrogenase
- In vivo electron transfer aldehyde oxidase
- Reactive oxygen species
- Substrates of XO - Oxidation
- Activation of Famciclovir by AO
- Uncharged N-heterocyclic substrates of AO
- Oxidative metabolism of Zaleplon
- Charged N-heterocyclic substrates of AO
- Aldehyde substrates of AO
- Human molybdenum hydroxylase activity
- Induction & inhibition of molybdenum hydroxylases
- AO and XO/XDH genes
- Variability in human liver aldehyde oxidase activity
- SNPs Xanthine Oxidase
- Genetic deficiencies in molybdenum hydroxylases
- ADH and ALDH
- Alcohol Dehydrogenase (ADH) (1)
- Alcohol Dehydrogenase (ADH) (2)
- Aldehyde oxidation
- Aldehyde Dehydrogenase (ALDH)
- Properties of ALDH
- Endogenous ALDH substrates
- ALDH polymorphism
- Amine oxidases
- Monoamine oxidase (MAO)
- Properties of MAO
- Flavin monooxygenases (FMO)
- FMO isozymes
- Catalytic cycle of FMO
- Substrate specificty of FMOs
- FMO oxidation of primary & secondary amines
- FMO oxidation of aliphatic tertiary amines
- Stereoselective oxidation of (S)-nicotine
- Sulphur containing drugs and xenobiotics
- Stereoselective S-oxidation of cimetidine
- FMO5 & Baeyer-Villiger oxidation
- Genetic polymorphism of FMO
- Inhibition and induction of FMO
- Lecture summary
- Thank you
Topics Covered
- Non-P450 cytosolic and mitochondrial enzymes catalysing drug oxidation
- Aldehyde oxidase and xanthine oxidase
- Alcohol dehydrogenase and aldehyde dehydrogenase
- Monoamine oxidase
- Flavin monooxygenase
- Substrate specificities
- Drug and xenobiotic substrates
- Catalytic mechanism and metabolite formation
- Reactive oxygen species
- Factors affecting enzyme activity
- Polymorphism and isozymes
- Relative importance in drug metabolism
Links
Series:
Categories:
Therapeutic Areas:
Talk Citation
Beedham, C. (2019, January 30). Non-P450 oxidative metabolism: characteristics and drug substrates [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 10, 2024, from https://doi.org/10.69645/JVOV1524.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Christine Beedham has no commercial/financial relationships to disclose.
Non-P450 oxidative metabolism: characteristics and drug substrates
Published on January 30, 2019
45 min
A selection of talks on Biochemistry
Transcript
Please wait while the transcript is being prepared...
0:00
Hello. My name is Dr. Christine Beedham,
and I'm an honorary visiting senior lecturer at the University of Bradford.
I'm going to talk about the Non-P450 enzymes which catalyze drug oxidation.
0:14
Although oxidated metabolism is generally linked
with the microsomal cytochrome P450 system,
oxidative reactions are not restricted to
cytochrome P450 or confined to the endoplasmic reticulum.
Cytosolic and mitochondrial enzymes
usually with complimentary substrate specificities to P450,
also contribute to drug a metabolite oxidation.
These enzymes include the molybdoflavoproteins or molybdenum hydroxylases,
aldehyde oxidase and xanthine oxidase,
NAD dependent aldehyde dehydrogenase and NAD dependent alcohol dehydrogenase,
and the flavoprotein monoamine oxidase.
These enzymes provide a very effective protection against xenobiotics,
as they catalyze the rapid oxidation of numerous nitrogen-containing hetrocycles,
aldehydes, alcohols, and amines.
Which is either independent of cellular co-factors or dependent on ubiquitous NAD+ .
Likewise, microsomal oxidation is not catalyzed exclusively by cytochrome P450.
Reactive nucleophiles undergo N and S oxidation by
the NADPH dependent flavin monooxygenases in liver and extra hepatic tissue.
1:33
This lecture will summarize the reactions catalyzed by these enzymes,
consider their substrate specificities and factors affecting their activity,
and thus their relative importance in the metabolism of drugs and xenobiotics.