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1. Intro slide
2. The RB pathway in human cancer
3. E2F responsive genes & timing of expression
4. Cell cycle regulation of a single promoter by E2F
5. E2F family members
6. E2F family members activator E2Fs
7. E2F family members repressor E2Fs
8. Pocket protein family members
9. E2F-RB family interactions
10. Chromatin immunoprecipitation (ChIP) strategy
11. E2F & pRB families: promoter occupancy
12. Histone acetylation parallels activation of E2F
13. HDAC binding during G0
14. HDAC recruitment in primary cells
15. E2F4/Sin3/HDAC promoter binding
16. E2F drives the transcriptional cycle
17. Cell cycle re-entry
18. Location analysis (ChIP on chip)
19. Repressor & activator E2F target genes overlap
20. Gene expression networks
21. Multiple targets de-regulated by p107/p130 loss
22. Distinct growth arrest conditions
23. Genes repressed upon cell cycle exit
24. Binding by E2F4 & p130: statistical correlations
25. p130-E2F4 represses a core set of genes
26. Over-expression of genes in primary tumors
27. Distinct growth arrest conditions
28. Identifying factors that cooperate with E2F
29. NRF1 binding to E2F responsive promoters
30. Knocking down a factor
31. How E2F controls the cell cycle
32. The cell cycle arrest network module
33. pRB family recruited to promoters in cycling cells
34. RB chIP on chip analysis
35. Distinct roles for the pRB family on chromatin
36. FACS analysis and western blot
37. E2F & pocket proteins roles
38. A histone acetylation/de-acetylation cycle
39. p107, p130, and E2F4: overlapping
40. E2F chIP on chip analysis
41. Expression analysis defines classes of E2F target
42. Sin3B marks promoters for repression in early G1
43. Promoter-specific de-acetylation
44. E2F drives the transcriptional cycle
45. Repressor E2F networks
46. E2F and RB and tumor suppression
47. E2F and apoptosis
48. Acknowledgements

Topics Covered

• Complex and diverse roles of E2F family members
• Interaction with tumour repressor proteins
• Interaction with chromatin modifying enzymes
• Study using microarray technology and chromatin immunoprecipitation
• Novel features of cell cycle dependent transcription
• Roles in regulating G1-to-S transition, mitosis, differentiation, and apoptosis

Links

Series:

• Eukaryotic Gene Regulation

Categories:

• Cancer
• Cell Biology
• Genetics & Epigenetics

Therapeutic Areas:

• Oncology

Talk Citation

Dynlacht, B. (2007, October 1). The E2F family and transcriptional control of the mammalian cell cycle [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved September 17, 2024, from https://doi.org/10.69645/NDKO9772.
Export Citation (RIS)

Publication History

• Published on October 1, 2007
• Archived on June 22, 2016

Financial Disclosures

• Prof. Brian Dynlacht has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.