• RNAi-Pathway Components and Mechanisms
• 39 min
  1. Activation of gene expression by double-stranded RNAs

  • Prof. Long-Cheng Li
• 31 min
  2. MicroRNA biogenesis

  • Dr. Narry Kim
• 35 min
  3. Dicer and R2D2

  • Dr. Young Sik Lee
• 26 min
  4. Mammalian microRNA assembly and function

  • Prof. Zissimos Mourelatos
• 33 min
  5. Mechanisms of RNAi: a molecular view or Argonaute: the secret life of Slicer

  • Prof. Leemor Joshua-Tor
• Roles for RNAi in Development and Anti-Viral Defense
• 38 min
  6. Functional roles of RNAi in C. elegans

  • Dr. Eric Miska
• 41 min
  7. RNAi and heterochromatin in plants and fission yeast

  • Prof. Robert Martienssen
• 29 min
  8. RNA interference functions and mechanisms in animals

  • Prof. Richard Carthew
• 40 min
  9. Specific functional roles in mammals

  • Dr. Michael McManus
• RNAi Design Rules for Applications in Humans
• 38 min
  10. siRNA on and off-target

  • Prof. Anastasia Khvorova
• 43 min
  11. Cell signalling pathways and RNAi

  • Mr. Michael O'Grady
• 33 min
  12. RNAi: key technological aspects

  • Dr. Dmitry Samarsky
  • Dr. Ciaran Faherty
• 28 min
  13. RNAi specificity: how big of an issue is it?

  • Dr. Peter Welch
• Applications of RNAi in the Treatment of Human Diseases
• 18 min
  14. siRNA therapeutics

  • Prof. John Rossi
  • Mr. Daniel J. Rossi
• 48 min
  15. RNAi for neurological diseases

  • Prof. Beverly L. Davidson
• 31 min
  16. RNA interference and cancer: a revolution?

  • Dr. Annick Harel-Bellan
• Archived Lectures *These may not cover the latest advances in the field
• 51 min
  17. RNAi and viral heart infections

  • Dr. Jens Kurreck
• 26 min
  18. RNA interference for neurodegenerative diseases

  • Prof. Beverly L. Davidson
• 42 min
  19. Activation of gene expression by double-stranded RNA

  • Prof. David Corey
• 43 min
  20. New designs for siRNAs as Dicer substrate

  • Dr. Mark Behlke
• 27 min
  21. RNAi in the model system Drosophila

  • Prof. Richard Carthew
• 29 min
  22. Small interfering RNA induced transcriptional gene silencing in human cells

  • Dr. Kevin Morris
• 37 min
  23. Therapeutic delivery of RNAi

  • Dr. Patrick Lu
• 44 min
  24. Mechanistic aspects and therapeutic applications of RNAi

  • Prof. John Rossi

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. RNA interference (RNAi)
3. RNAi: Post-Transcriptional Gene Silencing (PTGS)
4. RNAi: Transcriptional Gene Silencing (TGS)
5. dsRNA mediated TGS in S. Pombe
6. Do siRNAs directed to promoters induce TGS?
7. FIV lentiviral vector system
8. Experimental protocol
9. siRNA targeting EF1a promoter suppress GFP
10. Specificity of EF1A siRNA Mediated TGS
11. siRNA does not alter vector integration
12. siRNA mediated TGS is reversible
13. TGS is associated with DNA methylation
14. siRNA EF52 can target the EF1A promoter
15. EF1a is only inhibited at 48hrs post-transfection
16. Are intronic regions susceptible to siRNA targeting
17. Intronic region is susceptible to siRNA targeting
18. Specificity of EF315 transcriptional silencing
19. siRNA mediated TGS in human cells
20. What is the mechanism of siRNA mediated TGS?
21. TGS operates via chromatin remodeling pathway
22. siRNA mediated histone methylation
23. RNA pol II is required for siRNA mediated TGS
24. How do siRNAs find the target?
25. Detection of flag-tagged DNMTs or Hp1s
26. Specificity of DNMT binding
27. Antisense siRNA targeting HIV-1 LTR/promoter
28. Susceptibility of the HIV-1 LTR to siRNA
29. siRNA EF52 and all the factors are linked
30. Conclusions
31. Putative mechanism(s) for siRNA mediated TGS
32. Acknowledgements
33. Living on the edge

Topics Covered

• How siRNAs mediate transcriptional gene silencing (TGS) in human cells
• Histone methylation
• Dependency on active RNA polymerase II mediated transcription
• siRNA-mediated TGS
• H3K9 and H3K27 methylation of the targeted EF1A promoter
• Nuclear specific delivery of the siRNA
• Antisense strand-specific siRNA-mediated TGS of EF1A
• Targeting of the U3 region of the HIV-1 LTR/promoter
• Antisense strand of siRNA EF2 interacts with FLAG-tagged DNMT3A and the tri-methylated H3K27 methyl mark
• siRNA mediated targeting of genomic regions
• RNA Pol-II function
• Transcriptional gene silencing in human cells

Links

Series:

• RNA Interference

Categories:

• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on August 13, 2020

Financial Disclosures

• Dr. Kevin Morris has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.