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Printable Handouts
Navigable Slide Index
- Introduction
- Outline
- Gels and polymer networks
- Gel electrophoresis devices
- CGE system
- Interpretation of the results
- Detection modes in CGE
- Direct and indirect detection in CGE
- Laser-induced fluorescence detection
- MS detection with CGE
- Injection modes
- Problems with electrokinetic injection
- Injection related artifacts
- Selected electrophoretic sample preconcentration techniques
- Sample stacking in CGE
- Transient ITP sample preconcentration in CGE
- On-line immunoaffinity analyte concentrator-microreactor
- Effect of column coiling
- Temperature effects and power dissipation
- Capillary coating
- Theory
- The basic electric field mediated separation modes in gel filled capillaries
- Basic principles of CGE
- The reptation plot
- The Ferguson method in SDS-CGE of proteins
- Non-cross-linked polymer solutions (physical gels)
- The entanglement threshold
- Schematic representation of the presumed deformation of DNA
- Disclaimer
Topics Covered
- Detection modes in CGE (Capillary Gel Electrophoresis)
- Sample injection modes
- Pre concentration techniques in CGE
- Effect of column coiling
- Basic principles of CGE
- The reptation plot
- The Ferguson method
Talk Citation
Guttman, A. (2025, July 31). Capillary gel electrophoresis 1 [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved August 15, 2025, from https://doi.org/10.69645/CWCH7730.Export Citation (RIS)
Publication History
- Published on July 31, 2025
Financial Disclosures
- There are no commercial/financial matters to disclose.
A selection of talks on Pharmaceutical Sciences
Transcript
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0:00
Hi, everyone. My name
is András Guttman from
the Horváth Csaba Memorial
Laboratory of
Bioseparation Sciences
at the University of Debrecen,
Hungary and the University
of Pannonia, Veszprém,
Hungary as well,
giving a presentation about
capillary gel electrophoresis.
0:19
At the beginning of
the new millennium,
entering from the
age of genomics
into the age of
functional genomics
and other omics fields such
as proteomics,
glycomics, lipidomics,
metabolomics, etc,
we expect to see
high resolution separation
techniques used in
integrated and automated
fashion to solve
formidable separation
problems and
provide the support for
challenging analytical
applications such
as capillary gel
electrophoresis.
This presentation covers
the theoretical and
practical aspects
of capillary gel
electrophoresis,
and provides an overview of
the key application
areas of nucleic acid,
protein, and complex
carbohydrate analysis,
as well as affinity-based
methodologies.
1:03
This slide shows the gel
and polymer networks
being used in capillary
gel electrophoresis.
On the upper panels,
homogeneous and heterogeneous
gels are shown.
The homogeneous gel is
very well-defined
pore structure,
and the heterogeneous gels have
microgel formation
within this microgel.
On the lower panel, the
three mostly used gel types
in capillary gel
electrophoresis are shown.
The chemical gel,
covalent linking between
the cross-linked pair
and the monomer,
the physical gel,
in which case there are only
physical interactions
holding together the matrix,
and the transitionally
cross-linked type,
in which case in this example
the boric acid is holding
together the dextran chains.