Capillary gel electrophoresis 1

Published on July 31, 2025   31 min

A selection of talks on Pharmaceutical Sciences

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0:00
Hi, everyone. My name is András Guttman from the Horváth Csaba Memorial Laboratory of Bioseparation Sciences at the University of Debrecen, Hungary and the University of Pannonia, Veszprém, Hungary as well, giving a presentation about capillary gel electrophoresis.
0:19
At the beginning of the new millennium, entering from the age of genomics into the age of functional genomics and other omics fields such as proteomics, glycomics, lipidomics, metabolomics, etc, we expect to see high resolution separation techniques used in integrated and automated fashion to solve formidable separation problems and provide the support for challenging analytical applications such as capillary gel electrophoresis. This presentation covers the theoretical and practical aspects of capillary gel electrophoresis, and provides an overview of the key application areas of nucleic acid, protein, and complex carbohydrate analysis, as well as affinity-based methodologies.
1:03
This slide shows the gel and polymer networks being used in capillary gel electrophoresis. On the upper panels, homogeneous and heterogeneous gels are shown. The homogeneous gel is very well-defined pore structure, and the heterogeneous gels have microgel formation within this microgel. On the lower panel, the three mostly used gel types in capillary gel electrophoresis are shown. The chemical gel, covalent linking between the cross-linked pair and the monomer, the physical gel, in which case there are only physical interactions holding together the matrix, and the transitionally cross-linked type, in which case in this example the boric acid is holding together the dextran chains.

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