• Introduction to Protein Structure and Function
• 40 min
  1. Nature’s strategies in the regulation of enzyme activity by modifiers

  • Prof. Antonio Baici
• Creation of Protein Variability by Manipulation of Genes
• 28 min
  2. Understanding protein variability through functional analysis of random mutagenesis

  • Prof. Andrew Kaplan
• 48 min
  3. Perspectives on biological catalysis

  • Prof. Stephen Benkovic
• 45 min
  4. Fundamentals and principles for engineering proteolytic activity

  • Prof. Charles Craik
• Metabolic Diseases Caused by Genetic Mutation
• 36 min
  5. Modifications of pyruvate handling in health and disease

  • Prof. Mary Sugden
• 29 min
  6. Mitochondrial fatty acid oxidation deficiencies

  • Prof. Niels Gregersen
• 33 min
  7. Inborn errors of ketone body metabolism

  • Prof. Toshiyuki Fukao
• 34 min
  8. Cathepsin K in bone and joint diseases

  • Prof. Dieter Bromme
• 22 min
  9. Fabry disease: alfa-galactosidase A deficiency and enzyme replacement therapy

  • Prof. David Warnock
• 47 min
  10. Acid beta-glucosidase/glucocerebrosidase (GCase)

  • Prof. Gregory Grabowski
• 23 min
  11. GM2 gangliosidosis future treatments 1

  • Prof. Brian Mark
• 19 min
  12. GM2 gangliosidosis future treatments 2

  • Prof. Brian Mark
• 29 min
  13. The neuronal ceroid lipofuscinoses

  • Prof. Sandra Hofmann
• Disorders of Blood Coagulation
• 24 min
  14. Advances in fibrinolysis

  • Dr. Paul Kim
• 26 min
  15. Tissue factor and factor VII: initiation of blood coagulation

  • Dr. John McVey
• 36 min
  16. Structure of thrombin, a Janus-headed proteinase

  • Prof. Wolfram Bode
• 45 min
  17. The protein c-thrombomodulin mechanism: regulating multiple biological systems

  • Prof. Edward Conway
• 40 min
  18. Fibrinogen and factor XIII

  • Prof. John Weisel
• 31 min
  19. Factor VIII and haemophilia A

  • Dr. Geoffrey Kemball-Cook
• 34 min
  20. Factor IX

  • Prof. Bruce Furie
• 42 min
  21. The biology and pathobiology of von Willebrand factor

  • Prof. David Lillicrap
• 40 min
  22. Thrombotic thrombocytopenic purpura

  • Prof. J. Evan Sadler
• 26 min
  23. Fibrinolysis

  • Prof. Edward Tuddenham
• Other Molecular and Metabolic Disorders
• 45 min
  24. Glucose-6-phosphate dehydrogenase deficiency

  • Dr. Jane Leopold
• 26 min
  25. Cytochrome b5 reductase deficiency and hereditary methemoglobinemia

  • Dr. Scott Reading
• 50 min
  26. Sickle cell disease

  • Prof. Martin H. Steinberg
• 40 min
  27. Pyruvate kinase deficiency

  • Prof. Alberto Zanella
• 47 min
  28. Heritable disorders of collagen

  • Dr. Heather Yeowell
• 40 min
  29. Duchenne muscular dystrophy

  • Prof. Jeff Chamberlain
• Archived Lectures *These may not cover the latest advances in the field
• 33 min
  30. Protein crystallography

  • Prof. Michael James
• 48 min
  31. Regulation of blood coagulation by the serpin, antithrombin

  • Prof. Steve Olson
• 37 min
  32. Rhodopsin and retinitis pigmentosa

  • Dr. Shalesh Kaushal
• 40 min
  33. The physiology and pathology of coagulation factor XI

  • Dr. David Gailani
• 24 min
  34. Cytochrome b5 reductase deficiency and hereditary methemoglobinemia

  • Prof. Josef Prchal
• 49 min
  35. Metachromatic leukodystrophy

  • Prof. Volkmar Gieselmann
• 21 min
  36. Serpins and serpinopathies

  • Dr. James Whisstock
• 27 min
  37. Multiple acyl-CoA dehydrogenation deficiency: defects of electron transfer flavoproteins

  • Dr. Rikke Olsen
• 39 min
  38. Pleiotropic and epistatic genes in sickle cell anaemia

  • Prof. Ronald Nagel
• 44 min
  39. Genetic disorders of carbonic anhydrases II and IV

  • Prof. William Sly
• 39 min
  40. GM2 gangliosidoses

  • Prof. Don Mahuran
• 48 min
  41. Kinetic analysis of protein activity

  • Prof. Antonio Baici

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Fibrinogen important functions
3. Basic scheme of fibrin polymerization
4. The 3 pairs of polypeptide chains of fibrinogen
5. TEM fibrinogen images
6. Structure of fibrinogen
7. Biosynthesis of fibrinogen
8. Diagram of the complementary binding sites
9. Physical model of the protofibril
10. Structure of protofibrils
11. Schematic model of complementary binding sites
12. Activation of factor XIII
13. Formation of iso-peptide bonds in fibrin
14. Factor XIII stabilizes fibrin
15. Diagram of fibrin polymerization
16. Fibrin fibers structure in TEM
17. Specimen preparation for TEM: negative contrast
18. Twisting of protofibrils around each other
19. SEM of fibrin fibers images
20. Branch points of fibrin fibers
21. SEM of fibrin clots images (1)
22. The kinetics of polymerization
23. Fibrin is a viscoelastic polymer
24. The unique mechanical properties of fibrin
25. Fibrin clot formation: turbidity curves
26. Stiffness as another measure of polymerization
27. Another way to determine stiffness
28. Binding of other proteins to fibrin(ogen)
29. Light microscope images of fibrin clots
30. SEM of fibrin clots images (2)
31. Plasminogen activation and fibrin lysis
32. Fragments from plasmin digestion of fibrin
33. Electron micrographs of fibrinolysis
34. Products of fibrinolysis
35. Fibrin-plasmin interactions leading to cleavage (1)
36. Fibrin-plasmin interactions leading to cleavage (2)
37. Fibrinogen is necessary for platelet aggregation
38. TEM images of rotary shadowed alphaIIb-beta3
39. TEM images of fibrinogen-integrin complexes
40. Illustration of platelet aggregation
41. Dysfibrinogenemias
42. Fibrinogen variants
43. Evolution of fibrinogen
44. Summary

Topics Covered

• Fibrinogen and factor XIII
• Structure
• Biosynthesis
• Fibrin polymerization
• Crosslinking
• Clot mechanical properties
• Interactions with other proteins
• Fibrinolysis
• Platelet aggregation
• Dysfibrinogenemias and variants
• Evolution

Links

Series:

• Protein Epidemiology

Categories:

• Biochemistry
• Cell Biology

Therapeutic Areas:

• Haematology

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. John Weisel has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.