• Origins, Diversity and Persistence
• 51 min
  1. Plastid structure: development and differentiation

  • Dr. Kevin Pyke
• 43 min
  2. Origin and molecular evolution of the plastid

  • Prof. Debashish Bhattacharya
• 50 min
  3. The chloroplast genome and chloroplast gene expression

  • Prof. Christopher Howe
• 41 min
  4. Regulation of chloroplast gene expression: light activated translation

  • Prof. Stephen Mayfield
• 41 min
  5. Protein synthesis in plastids

  • Dr. William Zerges
• 43 min
  6. The chloroplast proteome

  • Dr. Julian Whitelegge
• 35 min
  7. Protein import into chloroplasts

  • Dr. Ute Vothknecht
• 45 min
  8. Assembly of the photosynthesis apparatus

  • Prof. J. Kenneth Hoober
• 26 min
  9. Plastid senescence

  • Prof. Howard Thomas
• Biosynthetic Support of the Biosphere
• 40 min
  10. Photosynthesis: energy capture

  • Prof. Robert Blankenship
• 27 min
  11. Photosynthesis: carbon assimilation

  • Prof. Richard Leegood
• 21 min
  12. Regulation of photosynthetic carbon metabolism

  • Prof. Louise Anderson
• 42 min
  13. Pigments: chlorophyll and its synthesis

  • Prof. Wolfhart Rudiger
• 34 min
  14. Carotenoid functions and other isoprenoids in plants

  • Prof. Elisabeth Gantt
• 30 min
  15. Chloroplast lipid biosynthesis

  • Prof. Christoph Benning
• 31 min
  16. Amino acid synthesis in chloroplasts

  • Prof. Peter Lea
  • Prof. Paulo Mazzafera
  • Dr. Ricardo Azevedo
• 53 min
  17. Responses of chloroplasts to stress conditions

  • Dr. Ron Mittler
• 67 min
  18. Chloroplast genetic engineering

  • Prof. Henry Daniell
• Archived Lectures *These may not cover the latest advances in the field
• 36 min
  19. The chloroplast genome and chloroplast gene expression

  • Prof. Christopher Howe

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Photosynthesis requires endomembrane systems
3. 2x glycolipids as phopholipids in Arabidopsis
4. Plant glycolipid: structures and analysis
5. Two chloroplast anionic lipids
6. Organic phosphorus bound in phospholipids
7. Glycolipids substitute phospholipids
8. Glycolipid to phospholipid ratio in pho1
9. Genetics to chloroplast lipid biosynthesis
10. Nucleotide pathway for SQDG biosynthesis
11. Sulfolipid biosynthesis in Rhodobacter sphaeroides
12. Recombinant AtSQD1
13. Sulfite is the sulfur donor
14. Proposed AtSQD1 reaction mechanism
15. 1.6 angstrom structure of AtSQD1
16. Active site of AtSQD1
17. Sulfolipid biosynthesis is a function of plastids
18. T-DNA disruption causes sulfolipid deficiency
19. Does lack of sulfolipid matter?
20. Reduced growth of sqd2 mutant under P stress
21. PG biosynthesis non-response to P stress in sqd2
22. Function of sulfolipid
23. Biosynthesis: sulfolipid vs. phosphatidylglycerol
24. PG functions beyond those of SQDG
25. Phenotype of a pgp1-1/sqd2 double mutant
26. Glycerolipids with galactose head groups
27. Galactolipids provided by MGD1 and DGD1
28. MGD1 is responsible for the bulk of MGDG
29. Lipid mutant screening by TLC
30. DGD1 is responsible for the bulk of DGDG
31. DGD1 encodes a galactosyl transferase
32. DGD1- independent pathway for DGDG
33. Second set of lipid galactosyltransferases
34. Expression of lipid genes induced by P stress
35. High-throughput screening for dgd1-suppressors
36. DGS1 encodes an integral membrane protein
37. Full-length DGS1-GFP localizes to mitochondria
38. DGS1-loop-GFP fusion localizes to nucleus
39. DGS1 control of MGD2 and MGD3 expression
40. DGS1 as part of a regulatory network
41. DGS1 regulation paradigm
42. Lipid trafficking in chloroplast biogenesis
43. Two dgd1-suppressor classes discovered
44. The tgd mutants have complex lipid phenotypes
45. Anomeric proton configuration of TGDG
46. GGGT activated in tgd1
47. Third galatosyltransferase type in tgd mutants
48. Oil droplets visible in tgd1 cytoplasm
49. Accumulation of TAGs in tgd mutant leaves
50. The tgd1 mutant accumulates phosphatidate
51. Two pathway hypothesis
52. Loss of the plastid pathway in act1 mutant
53. 16-C-to-18-C ratios - diagnostic for pathways
54. Disruptions in ER-plastid lipid transfer
55. Multipartite ABC-transporter in plastid envelopes
56. TGD1 associated with inner plastid envelope
57. Import of phosphatidate impaired in tgd1 mutant
58. TGD2 specifically binds phosphatidate
59. Role of the TGD in galactolipid biosynthesis
60. The tgd mutant phenotype
61. Chloroplast lipid biosynthesis: conclusions
62. Acknowledgments

Topics Covered

• Uniqueness of thylakoid membrane lipid composition
• Nonphosphorous glycerolipids
• Genetic analysis of lipid biosynthesis and function in Arabidopsis
• Regulation of membrane lipid composition by phosphate availability
• Biosynthesis of the plant sulfolipid
• Function of anionic lipids in the photosynthetic membrane
• Biosynthesis of galactoglycerolipids
• Lipid trafficking between the chloroplast and other organelles
• Regulation of lipid trafficking

Links

Series:

• Chloroplast

Categories:

• Biochemistry
• Plant & Animal Sciences

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Christoph Benning has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.