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1. Introduction
2. The critical role played by chlorophyll b
3. Main concepts included in this presentation
4. Part I: biology of development
5. Chloroplast in a young leaf of a tobacco plant
6. The structural feature of photosynthetic systems
7. Sketch of chloroplast development
8. Chloroplast development (1)
9. Chloroplast development (2)
10. Ultrastructure of cryofixed C. reinhardtii
11. The site of thylakoid membrane assembly (1)
12. The site of thylakoid membrane assembly (2)
13. Chlorophyll synthesis and LHCPs detection
14. Suppression of chlorophyll synthesis and LHCP
15. Chlorophyll b-deficient mutant of Arabidopsis
16. Cytosolic LHCPs accumulation
17. LHCP in polyphosphate granules
18. Part II: properties of the chlorophylls
19. Chlorophyll structures
20. Spectra of chlorophyll a and b in diethyl ether
21. Chlorophyll interacts with other molecule
22. Binding of Chl a to different imidazole groups
23. Ligands in synthetic maquettes of LHCP
24. Specificity determination of ligand selection
25. Chlorophyll structures - review
26. Excitation spectra of chlorophyll-peptide mixtures
27. Binding of chlorophylls to peptide
28. Electronegativity values for the elements H to K
29. Qy and Qx oscillator strengths of the chlorophylls
30. Structure of chlorophyll a
31. Electron cloud of chlorophyll a
32. Structure of chlorophyll b
33. Electron cloud of chlorophyll b
34. Structure of chlorophyll c
35. Chl a and d comparing to Chl b and c
36. Positions of the 14 Chl molecules in LHC II
37. Crystal structure of LHCII
38. LHCII requires three carotenoid molecules
39. Model of LHCII assembly
40. Part III: chlorophyllide a oxygenase
41. What is known about chlorophyll b synthesis?
42. Chlorophyll(ide) a oxygenase in C. reinhardtii
43. Chlorophyllide a oxygenase (CAO)
44. CAO immunoprecipitated analysis from plant cells
45. Arabidopsis CAO expression in E. coli
46. Iodide labeling of CAO
47. Iodide labels a conserved tyrosine
48. Part IV: photosynthesis as a probe
49. FRET assay for LHCII assembly
50. Freeze-fracture analysis of thylakoid membranes
51. Electron transport chain
52. Fluorescence induction curves
53. Fluorescence induction curve after 1 hr of greening
54. Summary
55. Reference (1)
56. Reference (2)

Topics Covered

• Biology of chloroplast development including biogenesis of the thylakoid membrane system
• Binding of chlorophylls to proteins determined by Lewis acid strength of the Mg
• Requirement for synthesis of chlorophyllide b on the inner membrane of the chloroplast envelope for import of apoproteins of light-harvesting complexes
• State transitions as a probe of thylakoid differentiation
• Initiation of thylakoid membrane assembly on the chloroplast envelope inner membrane

Links

Series:

• Chloroplast

Categories:

• Biochemistry
• Cell Biology
• Plant & Animal Sciences

Talk Citation

Publication History

• Published on October 1, 2007
• Reviewed on April 12, 2022

Financial Disclosures

• Prof. J. Kenneth Hoober has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.