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1. Introduction
2. General overview
3. Model organisms
4. Plastids
5. Overview: chloroplast ribosomes
6. Ribosomes: subunits and antibiotics sensitivities
7. In vivo pulse-labeling of Chlamydomonas proteins
8. Profile of ribosomes in sucrose gradient
9. Inheritance of streptomycin resistance
10. Ribosomes in wt and mutant: electron microscopy
11. Ribosomes: rRNAs
12. Chloroplast ribosomal RNAs
13. Chloroplast and bacteria ribosome
14. Ribosomes: rproteins
15. Protein composition on 2-D gel
16. Chloroplast ribosomal proteins (spinach)
17. pRRF - 70S chloroplast ribosome component
18. Chloroplast ribosomal proteins in C.reinhardtii
19. Overview: initiation
20. Initiation in eukaryotic cytoplasm
21. Initiation in bacteria
22. Initiation in chloroplasts (1)
23. Initiation in chloroplasts (2)
24. C. reinhardtii vs. vascular plants chloroplasts
25. Biochemical details are not all defined
26. Hirose and Sugiura research (1)
27. Hirose and Sugiura research (2)
28. Site directed mutagenesis and reporter genes
29. Cimeric reporter gene
30. Structural element is required for translation
31. Overview: elongation
32. Elongation details
33. Overview: termination
34. Termination details
35. Frequency of use of stop codons
36. Overview: membrane targeting
37. Targeting thylakoid proteins during their translation
38. Electron micrograph
39. Membrane targeting
40. Overview: summary
41. References: reviews
42. References: chloroplast ribosomes
43. References: the initiation phase
44. References: the elongation and termination phases
45. References: membrane targeting of ribosomes

Topics Covered

• Plastids have complete protein synthesis machinery
• Most plastid ribosomal proteins, ribosomal RNAs, translation factors and cisacting translational regulatory elements are homologous to components of bacterial protein synthesis
• Plastid-specific components have been identified
• Proteins are targeted to thylakoid membranes during their synthesis by plastid ribosomes

Links

Series:

• Chloroplast

Categories:

• Biochemistry
• Cell Biology
• Plant & Animal Sciences

Talk Citation

Publication History

• Published on October 1, 2007
• Reviewed on May 1, 2020

Financial Disclosures

• Dr. William Zerges has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.