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1. Introduction
2. Light is a key stimulus for plant development
3. Photosynthetic complexes
4. Nuclear and chloroplast gene expression
5. Chloroplast genome
6. Modulating translation in response to light
7. Nuclear mutations eliminate chloroplast translation
8. Nuclear encoded factors
9. Specificity of chloroplast RNA binding proteins
10. psbA RNA binding in response to light
11. Translational factors bind RNA in response to light
12. Oxidation/reduction of psbA RNA binding proteins
13. Oxidation and reduction by thioredoxin
14. Isolation of mRNA specific binding proteins
15. RB70 and RB38 form a stable complex
16. RB47 is a poly A binding protein
17. Oxidation/reduction of cPABP cystein mutant
18. Oxidaion/reduction of double mutant of cPABP
19. RB60 is a protein disulfide isomerase
20. Activation of cPAB1 by cPDI
21. Inactivation of cPAB1 in the presence of cPDI
22. Light activated translation of chloroplast mRNAs
23. Lack of psbA translation in hf261
24. TAD1 is an oxido-reductase
25. hf261 and tad1 gene restores acumulation of D1
26. hf261 & tad1 gene restores ribosome association
27. hf261 and tad1 gene restores psbA binding activity
28. TAD1 required for reduction of RB47
29. RNA elements function in translational regulation
30. Alignment of 3' 16S rRNA with 5' psbA mRNA
31. Protein synthesis in 16S SD mutants
32. SD position alteration mutants
33. Luciferase expression in SD spacing mutants
34. Translation initiation in chloroplast and bacteria
35. Impact of different 5' UTRs and 3' UTRs
36. Accumulation of chimeric GFP mRNAs
37. GFP accumulation in transgenic lines
38. The 5' UTR determines protein expression levels
39. Light regulated translation and translation rate
40. psbA-reporter gene replacement
41. Replacement of the psbA gene with psbA-SAA (1)
42. Replacement of the psbA gene with psbA-SAA (2)
43. Replacement of the psbA gene with psbA-SAA (3)
44. Counter balancing redox activated translation
45. Chloroplast and bacterial ribosomal proteins
46. Mechanism for translation initiation
47. Structure of chloroplast ribosomes
48. C. reinhardtii and E. coli 70S ribosomes with tRNA
49. 70S ribosomes with mRNA and tRNA
50. 3-D tanslational regulation

Topics Covered

• Chloroplast gene expression is primarily regulated during translation
• Light activates translation via a redox cascade initiated by photosynthetic electron transport
• Plastid specific translation factors and RNA elements interact with chloroplast ribosomes to facilitate translation initiation

Links

Series:

• Chloroplast

Categories:

• Biochemistry
• Cell Biology
• Genetics & Epigenetics
• Plant & Animal Sciences

Talk Citation

Mayfield, S. (2007, October 1). Regulation of chloroplast gene expression: light activated translation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/NTJR1618.
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Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Stephen Mayfield has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.