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Printable Handouts
Navigable Slide Index
- Introduction
- RTKs activate the ERK1/2 cascade
- Ras mediates activation of the ERK 1/2 cascade
- MAP kinase cascade paradigm
- Multiple MAP kinase pathways in mammalian cells
- Properties of MAPK cascades
- MAPK cascade
- Activation of Raf-1
- MEK1
- Structure of ERK2
- Effect of phosphorylation on ERK2
- Prop. of MAPK cascades-specificity generation
- ERK1/2 assays
- Enzymatic specificity of MEKs
- Function of MEK1
- Importance of proline-rich insert in MEK1
- Activation of ERK1/2 in cells requires MEK1 insert
- MEK1 binds stably to ERK2
- Recognition sites on ERK2
- MEK1 - function of ERK docking site in cells
- Docking interactions required for ERK2 phosphor.
- ERK2 interactions
- Mutations that decrease ERK2 activation by MEK1
- Behavior of ERK2 MEK-binding mutants
- Recognition sites on ERK2
- ERK1/2 scaffolding proteins direct distinct outputs
- KSR scaffolds the ERK cascade
- MEKK1 scaffolds MAPK cascades (1)
- MEKK1 scaffolds MAPK cascades (2)
- Properties of MAPK cascades
- Threshold and ultrasensitivity in ERK1/2 pathway
- Analysis of ERK2 from cells
- Signal transduc. mechanisms-coincidence/synergy
- Properties of MAPK cascades - signal modulation
- Feedback phosphorylation of SOS
- Activation of Raf-1
- Signal transduction mechanisms-feedback
- Properties of MAPK cascades-localization
- Ligand-dependent diff. in ERK1/2 localization
- ERK2 function depends on localization
- Classical nuclear import and export
- How does ERK2 enter and exit the nucleus?
- pERK2 dimer interface
- PEA-15 - protein enriched in astrocytes of 15 kDa
- PEA-15 reduces nuclear import of GFP-ERK2
- PEA-15 inhibits binding of ERK2 to nucleoporins
- Localization of ERK1/2 influences functions
- Properties of MAPK cascades - functions
- ERK1/2 are required for cell migration
- ERK1/2 required for insulin gene transcription (1)
- ERK1/2 required for insulin gene transcription (2)
- Properties of MAPK cascades - multifunctionality
- Properties of MAPK cascades
- Signal transduction mechanisms - modulation
- ERK1/2 sensitivity to cAMP depends on act. ligand
- ERK1/2 and ERK5 synergize to transform cells
- Differential action of cAMP on ERK1/2 and ERK5
- RTKs activate the ERK1/2 cascade
- Acknowledgements
Topics Covered
- Regulation of the MAPK pathway by receptor tyrosine kinases through Ras
- MAPK cascade paradigm
- Regulatory properties of kinase cascade components
- MAPK structure
- Generating specificity in MAPK cascades
- Docking mechanisms
- MAPK scaffolds
- Feedback mechanisms and pathway interactions
- Regulation of the localization of ERK1/2
- Functional versatility of MAPK pathways
Links
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Talk Citation
Cobb, M.H. (2007, October 1). RTKs activate the ERK 1/2 MAPK cascade [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 12, 2024, from https://doi.org/10.69645/FHGG9447.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Melanie H. Cobb has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.