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1. Introduction
2. Macromolecular crowding and protein aggregation
3. Topics to be discussed
4. The principle of protein self-assembly
5. Protein binding of a newly synthesized polypeptide
6. Rubisco chloroplast protein
7. Rubisco large subunit binding protein
8. Discovery of the chaperonins
9. BiP binding of the heavy chain of immunoglobulin
10. Origins of the molecular chaperone concept (1)
11. Origins of the molecular chaperone concept (2)
12. The march of molecular chaperones
13. The principle of protein self-assembly
14. In vivo protein folding comparing to in vitro folding
15. Protein aggregation
16. A possible fate for all polypeptide
17. Two key properties
18. Macromolecular crowding
19. Major consequences of macromolecular crowding
20. The excluded volume effect
21. The effect of macromolecular crowding
22. Effects of crowding on reaction rate
23. Predicted effects of macromolecular crowding
24. Effect of crowding agents on refolding of lysozyme
25. Crowding promotes the formation of amyloid fibrils
26. Lysozyme avoid aggregation when folding in ER
27. The solution to aggregation- in small chaperons
28. The solution to aggregation- in large chaperons
29. Molecular chaperones
30. The molecular chaperone function

Topics Covered

• The principle of protein self-assembly
• Discovery of proteins binding to newly synthesized polypeptides
• Discovery of the chaperonins
• Origins of the molecular chaperone concept
• Replacement of spontaneous self-assembly by assisted self-assembly
• Protein aggregation as a universal cellular problem
• Macromolecular crowding and its effects on reaction rates and association constants
• Stimulation of aggregation by crowding
• How chaperones combat aggregation
• Definition of molecular chaperones
• The chaperone function

Links

Series:

• Protein Folding, Aggregation and Design

Categories:

• Biochemistry
• Methods

Talk Citation

Ellis, R.J. (2007, October 1). Protein folding Inside the cell: macromolecular crowding and protein aggregation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 22, 2024, from https://doi.org/10.69645/FKHH1286.
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Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Emeritus R. John Ellis has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.