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Navigable Slide Index

1. Introduction
2. Concepts in protein folding
3. Why study single molecules?
4. Single molecules
5. Development of single molecule methods
6. Forster resonance energy transfer (FRET)
7. Observing protein folding with FRET
8. Confocal fluorescence detection
9. The spectroscopic ruler
10. End-to-end distance distributions
11. Dynamics and averaging regimes (1)
12. Dynamics and averaging regimes (2)
13. Equilibrium collapse
14. Single molecule protein folding kinetics
15. Equilibrium collapse summary
16. Dynamics from broadening
17. Unfolded chain dynamics
18. Estimating free energy barriers
19. Two-state folding of individual proteins (1)
20. Two-state folding of individual proteins (2)
21. Conclusions
22. Acknowledgements
23. Further reading

Topics Covered

• Concepts in protein folding
• Why study single molecules?
• Development of single molecule methods
• Forster Resonance Energy Transfer (FRET)
• Observing Protein Folding with FRET
• Two-colour confocal fluorescence detection
• The spectroscopic ruler
• End-to-end distance distributions
• Dynamics and averaging regimes
• Equilibrium collapse of an unfolded protein
• Single molecule protein folding kinetics
• Estimating free energy barriers for folding
• Two-state folding of individual protein molecules

Links

Series:

• Protein Folding, Aggregation and Design

Categories:

• Biochemistry

Talk Citation

Schuler, B. (2007, October 1). Protein folding and dynamics from single molecule spectroscopy [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 23, 2024, from https://doi.org/10.69645/MARF4356.
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Publication History

• Published on October 1, 2007
• Archived on April 17, 2016

Financial Disclosures

• Prof. Dr. Benjamin Schuler has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.