• Introduction to Protein Folding and Misfolding
• 69 min
  1. Protein folding and misfolding: from theory to therapy

  • Prof. Christopher Dobson
• Stability and Kinetics of Protein Folding
• 55 min
  2. Mechanisms of protein folding reactions

  • Prof. Thomas Kiefhaber
• Protein Folding Theory
• 53 min
  3. Mapping disordered proteins with single-molecule FRET

  • Dr. Hagen Hofmann
• Protein Folding Simulations
• 36 min
  4. Protein folding

  • Prof. Eugene Shakhnovich
• 42 min
  5. Simulating protein folding with full atomistic detail

  • Prof. Vijay Pande
• 58 min
  6. Molecular dynamics simulations of protein dynamics, unfolding and misfolding

  • Prof. Valerie Daggett
• Protein Folding Inside the Cell: Chaperones
• 42 min
  7. Protein folding Inside the cell: macromolecular crowding and protein aggregation

  • Prof. Emeritus R. John Ellis
• 34 min
  8. Chaperone mechanisms in cellular protein folding

  • Prof. Dr. F. Ulrich Hartl
• 60 min
  9. Quality control of proteins mislocalized to the cytosol

  • Dr. Ramanujan Hegde
• Protein Misfolding and Disease
• 44 min
  10. Protein aggregation, metals and oxidative stress in neurodegenerative diseases

  • Prof. David Allsop
• Protein Design
• 33 min
  11. Designing proteins with life sustaining activities 1

  • Prof. Michael Hecht
• 24 min
  12. Designing proteins with life sustaining activities 2

  • Prof. Michael Hecht
• 48 min
  13. Folding and design of helical repeat proteins

  • Prof. Lynne Regan
• 44 min
  14. Design and engineering of zinc-finger domains

  • Prof. Jacqui Matthews
• 47 min
  15. Prediction and design of protein structures and interactions

  • Prof. David Baker
• Amyloid Fibrils: Structure, Formation and Nanotechnology
• 39 min
  16. Amyloid fibrils as functional nanomaterials

  • Prof. Juliet Gerrard
• 37 min
  17. Functional amyloid fibrils from fungi and viruses

  • Prof. Margaret Sunde
• Intrinsically disordered Proteins
• 40 min
  18. Fuzzy protein theory for disordered proteins

  • Prof. Monika Fuxreiter
• Intersection of RNA, translation and protein aggregation.
• 47 min
  19. Expanding roles of RNA-binding proteins in neurodegenerative diseases

  • Prof. Aaron D. Gitler
• Proteostasis
• 49 min
  20. Adapting proteostasis to ameliorate aggregation-associated amyloid diseases

  • Dr. Jeffery W. Kelly
• Archived Lectures *These may not cover the latest advances in the field
• 60 min
  21. Amyloidosis: disease caused by amyloid

  • Prof. Mark Pepys
• 34 min
  22. Protein folding and dynamics from single molecule spectroscopy

  • Prof. Dr. Benjamin Schuler
• 41 min
  23. Prion diseases

  • Prof. Fred Cohen
• 31 min
  24. Basic studies of protein folding and stability: the folding of related protein families

  • Dr. Jane Clarke
• 38 min
  25. Titin I27: a protein with a complex folding landscape

  • Dr. Jane Clarke
• 49 min
  26. Novel proteins from designed combinatorial libraries

  • Prof. Michael Hecht
• 47 min
  27. Fast folding dynamics of small proteins: placing limits on diffusional limits

  • Dr. Stephen Hagen
• 35 min
  28. The sequence determinants of amyloid fibril formation

  • Prof. Fabrizio Chiti

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Some basic time scales in folding (1)
3. Loop formation times
4. Chain relaxation times
5. Some basic time scales in folding (2)
6. Folding as a unimolecular reaction
7. Classical transition state theory (TST)
8. Folding is diffusion-controlled
9. Diffusion driven barrier-crossing
10. Kramers behavior in folding simulations
11. Viscosity dependence of DNA hairpin closure
12. Folding: is friction due to solvent alone?
13. Solvent viscosity and peptide loop formation
14. Internal friction and polymer dynamics
15. Internal friction due to backbone rotations
16. Internal friction in conformational dynamics
17. Viscosity effects in protein folding
18. Other complications in viscosity studies
19. Internal friction in folding? (I)
20. Internal friction in folding? (2)
21. Internal friction in folding? (3)
22. Preparing a "friction-limited" system
23. Laser-triggered folding kinetics
24. Folding time vs. solvent viscosity
25. Comparing two IF experiments
26. Reaction-coordinate dependent diffusion constant
27. Large activation energy for t-0
28. Tryptophan Cage (Tc5b)
29. Viscosity dependence at constant delta G
30. TrpCage k-f and k-u
31. Compare to k-f simulations
32. Limiting folding/unfolding times
33. Conclusions
34. Acknowledgments

Topics Covered

• Basic time scales and diffusional limits in protein folding
• Applying reaction rate theories to folding
• Kramers behavior and friction
• Internal friction of polymer chains
• Evidence for (or against) internal friction in protein folding
• Fast folding experiments and methodology
• Interpretations: internal time scales versus internal viscosity
• Limitations of the "diffusional limits" picture

Links

Series:

• Protein Folding, Aggregation and Design

Categories:

• Biochemistry

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on August 13, 2020

Financial Disclosures

• Dr. Stephen Hagen has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.