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Navigable Slide Index

1. Introduction
2. The protein folding problem
3. Thermodynamics
4. Measuring stability
5. Measuring stability using denaturant
6. Stability and unfolding rate of proteins
7. Monitoring of protein folding
8. Measuring change of stability on mutation
9. Kinetics
10. Protein folding landscapes
11. Measuring kinetics
12. Why are ku and kf denaturant dependent
13. The Chevron plot
14. Slopes of the chevron plot and TS structure level
15. Protein engineering - a powerful tool
16. Mapping the transition state - Phi-value analysis
17. Phi-value analysis - free energy dependence
18. Determining changes in free energy
19. Phi value analysis - Phi value = 1
20. Phi value analysis - Phi value = 0
21. Phi value analysis - 0 < Phi < 1
22. Folding of related families of proteins
23. SCOP
24. Folding pathways - constraint by structure
25. Titin I27
26. TI I27 folds via a low energy intermediate
27. TI I27 mutants - Chevron plots
28. Phi value analysis of TI I27
29. Folding rates of other Ig-like proteins
30. All Ig proteins have similar Phi values
31. TI I27 - sturctured TS, TNfn3 - less structured TS
32. TI I27 and spectrin - like proteins
33. TI I27 and spectrin-like proteins - comparison
34. TI I27 and spectrin-like proteins - difference
35. Alpha spectrin-like - folding
36. Folding simulation
37. TI I27 and spectrin-like proteins - conclusion
38. Folding of related protein families - conclusion
39. Acknowledgements

Topics Covered

• The basics of protein folding studies: protein stability (thermodynamics) and protein folding kinetics
• The shape and interpretation of simple chevron plots
• How protein engineering phi value analysis can be used to investigate the structure of the transition state for protein folding
• How studies of the folding of related proteins can simplify the problem of investigating how proteins fold
• Comparison of the folding of complex, all-beta immunoglobulin-like proteins to the folding of simple 3-helix bundles

Links

Series:

• Protein Folding, Aggregation and Design

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Clarke, J. (2007, October 1). Basic studies of protein folding and stability: the folding of related protein families [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/DUDE7612.
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Publication History

• Published on October 1, 2007
• Archived on April 17, 2016

Financial Disclosures

• Dr. Jane Clarke has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.