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1. Introduction
2. Outline of lecture
3. What are Ca waves and oscillations?
4. Ca waves
5. Ca oscillations
6. Ca transients encodes important information
7. Changes in intracellular Ca signaling
8. Introduction to Ca signaling
9. What is needed for intracellular Ca signaling?
10. InsP3R type I in the cerebellar purkinje cells
11. InsP3R: three isoforms and three domains
12. InsP3R: a large protein that makes an ion channel
13. InsP3R production is required
14. The InsP3 dependence varies among isoforms
15. Ca dependence of the InsP3R isoforms
16. Signal initiation in dendrites
17. Chromagranin A and B bind to the InsP3R
18. Ca/InsP3R regulate channel activity
19. Effect of chromagranin B on InsP3R activity
20. CGB fragment alters InsP3R channel function
21. CGB fragment alters Ca release in PC12 cells
22. The localization of CGB is not uniform in cells
23. Many other proteins are uniformly distributed
24. The functional coupling of CGB to the InsP3R
25. CGB localization in hippocampus rat cells
26. Disease-related changes in Ca signaling
27. Conclusions: InsP3R and chromogranin
28. Signal initiation in the cytoplasm
29. Testosterone induced Ca oscillations
30. The importance of buffers
31. Ca buffers expression in specific regions of the cell
32. Testosterone induced Ca oscillations
33. Testosterone induced neurite outgrowth
34. High testosterone decreases cell viability
35. High testosterone changes the calcium signal
36. Conclusions: InsP3R and testosterone
37. Regulation of oscillations
38. SHP-2: SH2 domain-containing PTP
39. SHP-2 positively regulates RTK pathways
40. SHP-2 is required for FGF-induced Ca oscillations
41. Noonan's syndrome
42. NS-SHP-2 mutation enhances Ca oscillation
43. SHP2 modulates Ca oscillation in cardiac myocyte
44. Frequency of Ca oscillations and NFAT activity
45. Conclusions InsP3R and SHP-2
46. Conclusions
47. Collaborators

Topics Covered

• Calcium as a trigger for many cellular events including muscle contraction, hormone secretion and cell growth
• The release of calcium from intracellular stores using electrophysiological, biochemical and molecular techniques combined with imaging
• Regulation by chromogranin, a protein that binds to the major calcium release channel, the InsP3R
• Regulation by a steroid hormone both at physiological and supra-physiological levels
• Regulation by a protein tyrosine phosphatase SHP-2
• The consequences of mutations or non-physiological modulations of each pathway

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Ehrlich, B. (2007, October 1). Regulation of intracellular calcium signaling, localized signals and oscillations [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/RSGR4973.
Export Citation (RIS)

Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Barbara Ehrlich has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.