Registration for a live webinar on 'Precision medicine treatment for anticancer drug resistance' is now open.
See webinar detailsWe noted you are experiencing viewing problems
-
Check with your IT department that JWPlatform, JWPlayer and Amazon AWS & CloudFront are not being blocked by your network. The relevant domains are *.jwplatform.com, *.jwpsrv.com, *.jwpcdn.com, jwpltx.com, jwpsrv.a.ssl.fastly.net, *.amazonaws.com and *.cloudfront.net. The relevant ports are 80 and 443.
-
Check the following talk links to see which ones work correctly:
Auto Mode
HTTP Progressive Download Send us your results from the above test links at access@hstalks.com and we will contact you with further advice on troubleshooting your viewing problems. -
No luck yet? More tips for troubleshooting viewing issues
-
Contact HST Support access@hstalks.com
-
Please review our troubleshooting guide for tips and advice on resolving your viewing problems.
-
For additional help, please don't hesitate to contact HST support access@hstalks.com
We hope you have enjoyed this limited-length demo
This is a limited length demo talk; you may
login or
review methods of
obtaining more access.
- Introduction to Calcium Signaling
-
1. Introduction to cellular calcium signaling
- Dr. Martin Bootman
-
2. Monitoring Ca2+ concentration in living cells
- Dr. Marisa Brini
-
3. Cell boundary theorem
- Prof. Eduardo Ríos
- Calcium Influx
-
4. Arachidonic acid and store-independent Ca2+ entry
- Dr. Luca Munaron
-
5. Voltage-dependent calcium channels
- Prof. Annette Dolphin
-
7. Intracellular Ca2+ signaling: calcium influx
- Prof. Anant Parekh
-
8. Molecular identification of the CRAC channel
- Prof. Michael Cahalan
- Calcium Release
-
10. The InsP3 receptor calcium release channel
- Prof. J. Kevin Foskett
-
11. Molecular biology of ryanodine receptors: an overview
- Dr. Christopher George
- Prof. F. Anthony Lai
-
12. cADPR and NAADP: messengers for calcium signalling
- Prof. Antony Galione
-
13. Ryanodine receptors and cardiac function
- Prof. David Eisner
- Calcium Efflux and Sequestration
-
14. Sodium-calcium exchange
- Prof. John Reeves
- Organelle Calcium
-
15. Regulation and role of mitochondrial Ca2+ homeostasis
- Prof. Rosario Rizzuto
-
16. Peroxisomes and Golgi apparatus as players in Ca2+ homeostasis
- Dr. Paola Pizzo
- Dr. Alex Costa
-
17. Ca2+ dynamics between mitochondria and the endoplasmic reticulum
- Dr. Wolfgang Graier
-
18. Nuclear calcium signaling
- Dr. Oleg Gerasimenko
- Dr. Julia Gerasimenko
- Spatiotemporal Calcium Signals
-
19. Regulation of intracellular calcium signaling, localized signals and oscillations
- Prof. Barbara Ehrlich
- Calcium Effectors
-
24. Calcium-regulated adenylyl cyclases and cyclic AMP compartmentalization
- Prof. Dermot Cooper
-
25. Calcium and transcription-coupling
- Dr. Karen Lounsbury
-
26. Cellular calcium (Ca2+) buffers
- Prof. Dr. Beat Schwaller
-
27. Extracellular calcium signaling
- Dr. Aldebaran M. Hofer
-
28. Ca2+, fertilization and egg activation
- Prof. Karl Swann
-
29. Calcium regulation of transcription in plants
- Prof. Hillel Fromm
-
30. Mechanisms regulating STIM expression and function in Ca2+ signaling
- Dr. Jonathan Soboloff
-
31. Dynamic signal encoding in the S. cerevisiae calcium response
- Dr. Chiraj Dalal
- Calcium and Disease
-
32. Polycystins, calcium signaling and pathogenesis of polycystic kidney disease
- Prof. Laura del Senno
-
33. Ca2+ alterations in familial Alzheimer's disease (FAD)
- Dr. Paola Pizzo
-
34. Pancreatitis and calcium signaling
- Prof. Ole Petersen
-
35. Mechanism-based therapies for heart failure and cardiac arrhythmias
- Prof. Andrew Marks
-
36. Genetic defects and calcium
- Prof. Tullio Pozzan
- Archived Lectures *These may not cover the latest advances in the field
-
37. Calcium, calmodulin and calcineurin
- Prof. Stephen Bolsover
-
38. Calcium flickers steer cell migration
- Prof. Heping Cheng
-
39. Automated Ca2+ imaging of chemosensory neurones in C.elegans
- Dr. Nikos Chronis
-
40. Ca2+ and the regulation of small GTPases
- Prof. Peter Cullen
-
41. Genetically encoded Ca2+ indicators: molecular scale measurements in mammals in vivo
- Dr. Michael I. Kotlikoff
-
42. Capacitative (store-operated) calcium entry
- Dr. Jim Putney
-
43. The molecular biology of the inositol trisphosphate receptor
- Dr. Randen Patterson
-
44. Coordinated Ca2+ release from intracellular Ca2+ stores
- Prof. Ole Petersen
-
45. The plasma membrane calcium pump: biochemistry, physiology and molecular pathology
- Prof. Ernesto Carafoli
-
46. The calcium saga: a matter of life and death
- Prof. Pierluigi Nicotera
-
47. Ca2+ efflux and Ca2+ signals
- Dr. Anne Green
-
50. Modeling Ca2+ signals
- Dr. David Friel
Printable Handouts
Navigable Slide Index
- Introduction
- Biophysics of Ca signaling
- Extracellular and intracellular calcium sources
- Diffusion limits the spread of Ca ions in a cell
- Ca diffusion in the cytosol is slowed by Ca buffers
- Spatial aspects of Ca signaling
- Ca feedback on the IP3 receptor
- Ca induced Ca release propagates Ca waves
- Experimental methods
- Fluorescence microscopy
- Conventional fluorescence image of pollen grain
- Confocal sections through pollen grain
- Caged IP4
- Line-scanning confocal microscope - the system
- Line-scanning confocal microscope - results
- The experimental system: oocyte of X. laevis
- 'Elementary' calcium release events
- 'Local' Ca "puffs" in the Xenopus oocyte
- Linescan imaging of puffs
- Triggering of Ca waves by puffs
- Model of local and global IP3-evoked Ca signals
- The spread of Ca during elementary events
- Ca signals show a continuum of sizes
- Kinetics of puffs
- Puff occurrence is stochastic
- Saltatory propagation of Ca waves between puffs
- Triggering of calcium waves by puffs
- Method for rapidly changing [IP3] and [Ca] (1)
- Both photolysis and UV zap simultaneously
- Ca amount in a puff is too small to trigger a wave
- Role of puffs in setting frequency of Ca waves
- Puffs occur between, and trigger, Ca waves
- The regular periodicity of Ca waves
- Failure of puffs to trigger a wave
- Wave triggering depends on Ca accumulation
- Focal wave initiation sites are more IP3-sensitive
- Ca buffers affect coupling between puff sites
- Ca buffering proteins
- 'Slow' vs. 'fast' Ca buffers
- Conclusions
- Why are Ca channels organized in clusters?
- Acknowledgements
Topics Covered
- Calcium as a life and death cellular messenger
- Sequestration and liberation of calcium from the endoplasmic reticulum
- Calcium release through inositol trisphosphate receptors and modulation by calcium
- Optical techniques to visualize and stimulate intracellular calcium signals
- 'Elementary' calcium signals through single channels and clusters of channels
- Generation of periodic calcium waves by stochastic elementary events
- Modulation of calcium signals by calcium binding proteins
- The hierarchical organization of cellular calcium signals
Talk Citation
Parker, I. (2007, October 1). Spatial and temporal aspects of cellular calcium signals: 'elementary' signals and waves [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 12, 2024, from https://doi.org/10.69645/NYDX3531.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Ian Parker has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Spatial and temporal aspects of cellular calcium signals: 'elementary' signals and waves
A selection of talks on Biochemistry
Hide