Kinetic analysis of protein activity

Published on October 1, 2007 Reviewed on September 6, 2015   48 min

Other Talks in the Series: Protein Epidemiology

Ladies and gentlemen, dear colleagues. Antonio Baici from the University of Zurich. I guide you through an excursion in the world of kinetics, commented with Italian accent. The purpose of my talk is to discuss some topics and methods of practical interest for the kinetic investigation of proteins interacting with other proteins, ligands, and metabolites in general, with emphasis on the kinetics of enzyme action.
Biochemical kinetics deals with the study of the velocity of biologically relevant processes. It represents an indispensable support for understanding the mechanism of action of proteins. In fact knowing how fast an event takes place gives useful information about its character. We are aware that kinetics alone cannot answer all questions on the mechanism of action of the biological process. However, pulled information from kinetics, structured analyses, site-directed mutagenesis, and other methods will do the job.