• Introduction to Calcium Signaling
• 72 min
  1. Introduction to cellular calcium signaling

  • Dr. Martin Bootman
• 20 min
  2. Monitoring Ca2+ concentration in living cells

  • Dr. Marisa Brini
• 30 min
  3. Cell boundary theorem

  • Prof. Eduardo Ríos
• Calcium Influx
• 19 min
  4. Arachidonic acid and store-independent Ca2+ entry

  • Dr. Luca Munaron
• 34 min
  5. Voltage-dependent calcium channels

  • Prof. Annette Dolphin
• 34 min
  6. Protein networks of voltage-gated Ca2+ channels – proteomic & functional analysis of local Ca2+ signalling

  • Prof. Bernd Fakler
• 37 min
  7. Intracellular Ca2+ signaling: calcium influx

  • Prof. Anant Parekh
• 60 min
  8. Molecular identification of the CRAC channel

  • Prof. Michael Cahalan
• 35 min
  9. TRP channels with diverse permeability profiles: regulation of blood pressure and fertility

  • Prof. Marc Freichel
• Calcium Release
• 47 min
  10. The molecular biology of the inositol trisphosphate receptor

  • Dr. Randen Patterson
• 53 min
  11. The InsP3 receptor calcium release channel

  • Prof. J. Kevin Foskett
• 31 min
  12. Molecular biology of ryanodine receptors

  • Dr. Christopher George
  • Prof. F. Anthony Lai
• 57 min
  13. cADPR and NAADP: messengers for calcium signalling

  • Prof. Antony Galione
• 50 min
  14. Coordinated Ca2+ release from intracellular Ca2+ stores

  • Prof. Ole Petersen
• 43 min
  15. Ryanodine receptors and cardiac function

  • Prof. David Eisner
• Calcium Efflux and Sequestration
• 45 min
  16. The plasma membrane calcium pump: biochemistry, physiology and molecular pathology

  • Prof. Ernesto Carafoli
• 60 min
  17. The calcium saga: a matter of life and death

  • Prof. Pierluigi Nicotera
• 45 min
  18. Sodium-calcium exchange

  • Prof. John Reeves
• 22 min
  19. Ca2+ efflux and Ca2+ signals

  • Dr. Anne Green
• Organelle Calcium
• 42 min
  20. Regulation and role of mitochondrial Ca2+ homeostasis

  • Prof. Rosario Rizzuto
• 54 min
  21. Peroxisomes and Golgi apparatus as players in Ca2+ homeostasis

  • Dr. Paola Pizzo
  • Dr. Alex Costa
• 31 min
  22. Organellar calcium pumps of the golgi and ER: from yeast to human

  • Prof. Rajini Rao
• 34 min
  23. Ca2+ dynamics between mitochondria and the endoplasmic reticulum

  • Dr. Wolfgang Graier
• 26 min
  24. Nuclear calcium signaling

  • Dr. Oleg Gerasimenko
  • Dr. Julia Gerasimenko
• Spatiotemporal Calcium Signals
• 58 min
  25. Spatial and temporal aspects of cellular calcium signals: 'elementary' signals and waves

  • Prof. Ian Parker
• 54 min
  26. Regulation of intracellular calcium signaling, localized signals and oscillations

  • Prof. Barbara Ehrlich
• 26 min
  27. Modeling Ca2+ signals

  • Dr. David Friel
• Calcium Effectors
• 34 min
  28. Neuronal calcium sensor proteins: calcium sensors and regulators of diverse cellular functions

  • Prof. Bob Burgoyne
• 41 min
  29. Calcium-regulated adenylyl cyclases and cyclic AMP compartmentalization

  • Prof. Dermot Cooper
• 32 min
  30. Calcium and transcription-coupling

  • Dr. Karen Lounsbury
• 50 min
  31. Cellular calcium (Ca2+) buffers

  • Prof. Dr. Beat Schwaller
• 24 min
  32. Extracellular calcium signaling

  • Dr. Aldebaran M. Hofer
• 50 min
  33. Ca2+, fertilization and egg activation

  • Prof. Karl Swann
• 24 min
  34. Calcium regulation of transcription in plants

  • Prof. Hillel Fromm
• 34 min
  35. Mechanisms regulating STIM expression and function in Ca2+ signaling

  • Dr. Jonathan Soboloff
• 46 min
  36. Dynamic signal encoding in the S. cerevisiae calcium response

  • Dr. Chiraj Dalal
• Calcium and Disease
• 43 min
  37. Polycystins, calcium signaling and pathogenesis of polycystic kidney disease

  • Prof. Laura del Senno
• 28 min
  38. Ca2+ alterations in familial Alzheimer's disease (FAD)

  • Dr. Paola Pizzo
• 38 min
  39. Pancreatitis and calcium signaling

  • Prof. Ole Petersen
• 47 min
  40. Mechanism-based therapies for heart failure and cardiac arrhythmias

  • Prof. Andrew Marks
• 46 min
  41. Genetic defects and calcium

  • Prof. Tullio Pozzan
• Archived Lectures *These may not cover the latest advances in the field
• 31 min
  42. Calcium, calmodulin and calcineurin

  • Prof. Stephen Bolsover
• 34 min
  43. Calcium flickers steer cell migration

  • Prof. Heping Cheng
• 28 min
  44. Automated Ca2+ imaging of chemosensory neurones in C.elegans

  • Dr. Nikos Chronis
• 46 min
  45. Ca2+ and the regulation of small GTPases

  • Prof. Peter Cullen
• 35 min
  46. Genetically encoded Ca2+ indicators: molecular scale measurements in mammals in vivo

  • Dr. Michael I. Kotlikoff
• 35 min
  47. Capacitative (store-operated) calcium entry

  • Dr. Jim Putney

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Ca2+- modulated functions in mammalian cells
3. Ca2+ -binding proteins
4. Ca2+ -transporting proteins
5. Plasma membrane Ca2+ pump (PMCA pump)
6. PMCA pump - structure
7. PMCA pump - calmodulin binding
8. Activation of PMCA pump by calmodulin (CaM)
9. The four basic isoforms of PMCA pump
10. Main properties of the four basic pump isoforms
11. Splicing isoforms of PMCA pump
12. PMCA4 splicing isoforms: CaM affinity
13. PMCA3 splicing isoforms: CaM affinity
14. The isoforms interact with various partners
15. Interaction of PMCA with the 14-3-3-epsilon protein
16. The 14-3-3-epsilon proteins
17. PMCA4 pulls down only 14-3-3-epsilon
18. 14-3-3-epsilon overexpression or silencing
19. 14-3-3-epsilon overexpression: PMCA 2 vs. 4
20. Calcium signaling and disease
21. Reminder: splicing isoforms of PMCA pump
22. PMCA2 expression
23. The organ of Corti
24. Ca2+ modulate the current generated by hair cells
25. PMCA2 is expressed specifically in the stereocilia
26. Alternative splicing of PMCA2
27. Immunolocalization of different PMCA2 isoforms
28. Differences in the activity of PMCA2 isoforms
29. Effects of mutations in the PMCA2 gene
30. PMCA2wa mutations linked to deafness
31. Immunolocalization of PMCA2 mutants
32. Differences in the activity of mutated PMCA2 (1)
33. Differences in the activity of mutated PMCA2 (2)
34. Immunolocalization of mutated PMCA2 variants
35. Acknowledgements

Topics Covered

• Targets of the signaling function of calcium
• Decoding of the calcium message by specific calcium binding proteins
• Control of calcium in the cell by calcium transporting proteins in the membranes
• Calcium pumps: biochemistry, molecular biology, structure and regulation
• Similarities in the regulation mechanisms between the two major pumps, that in the endoplasmic reticulum and that in the plasma membrane
• Ambivalence of the calcium signal
• Calcium signaling defects and related pathologies
• Genetic defects of the plasma membrane calcium pumps in hereditary deafness

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology
• Clinical Medicine

Therapeutic Areas:

• Cardiovascular & Metabolic

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Ernesto Carafoli has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.