• Introduction to Calcium Signaling
• 72 min
  1. Introduction to cellular calcium signaling

  • Dr. Martin Bootman
• 30 min
  2. Cell boundary theorem

  • Prof. Eduardo Ríos
• Calcium Influx
• 19 min
  3. Arachidonic acid and store-independent Ca2+ entry

  • Dr. Luca Munaron
• 34 min
  4. Protein networks of voltage-gated Ca2+ channels – proteomic & functional analysis of local Ca2+ signalling

  • Prof. Bernd Fakler
• 35 min
  5. TRP channels with diverse permeability profiles: regulation of blood pressure and fertility

  • Prof. Marc Freichel
• Calcium Release
• 31 min
  6. Molecular biology of ryanodine receptors: an overview

  • Dr. Christopher George
  • Prof. F. Anthony Lai
• Organelle Calcium
• 54 min
  7. Peroxisomes and Golgi apparatus as players in Ca2+ homeostasis

  • Dr. Paola Pizzo
  • Dr. Alex Costa
• Spatiotemporal Calcium Signals
• 43 min
  8. Modeling Ca2+ signals: understanding Ca2+ regulatory networks in cells: introduction and basic concepts

  • Dr. David Friel
• 41 min
  9. Modeling Ca2+ signals: understanding Ca2+ regulatory networks in cells - Ca2+ stores: endoplasmic reticulum, calcium oscillations

  • Dr. David Friel
• 49 min
  10. Modeling Ca2+ signals: understanding Ca2+ regulatory networks in cells - Ca2+ stores: mitochondria, models of spatially non-uniform Ca2+ signals, applications to analysis of experimental results

  • Dr. David Friel
• Calcium Effectors
• 34 min
  11. Neuronal calcium sensor proteins: calcium sensors and regulators of diverse cellular functions

  • Prof. Bob Burgoyne
• 32 min
  12. Calcium and transcription-coupling

  • Dr. Karen Lounsbury
• 50 min
  13. Cellular calcium (Ca2+) buffers

  • Prof. Dr. Beat Schwaller
• 24 min
  14. Extracellular calcium signaling

  • Dr. Aldebaran M. Hofer
• 50 min
  15. Ca2+, fertilization and egg activation

  • Prof. Karl Swann
• 24 min
  16. Calcium regulation of transcription in plants

  • Prof. Hillel Fromm
• 34 min
  17. Mechanisms regulating STIM expression and function in Ca2+ signaling

  • Dr. Jonathan Soboloff
• 46 min
  18. Dynamic signal encoding in the S. cerevisiae calcium response

  • Dr. Chiraj Dalal
• Calcium and Disease
• 28 min
  19. Ca2+ alterations in familial Alzheimer's disease (FAD)

  • Dr. Paola Pizzo
• Archived Lectures *These may not cover the latest advances in the field
• 31 min
  20. Calcium, calmodulin and calcineurin

  • Prof. Stephen Bolsover
• 34 min
  21. Calcium flickers steer cell migration

  • Prof. Heping Cheng
• 28 min
  22. Automated Ca2+ imaging of chemosensory neurones in C.elegans

  • Dr. Nikos Chronis
• 46 min
  23. Ca2+ and the regulation of small GTPases

  • Prof. Peter Cullen
• 35 min
  24. Genetically encoded Ca2+ indicators: molecular scale measurements in mammals in vivo

  • Dr. Michael I. Kotlikoff
• 35 min
  25. Capacitative (store-operated) calcium entry

  • Dr. Jim Putney
• 47 min
  26. The molecular biology of the inositol trisphosphate receptor

  • Dr. Randen Patterson
• 50 min
  27. Coordinated Ca2+ release from intracellular Ca2+ stores

  • Prof. Ole Petersen
• 45 min
  28. The plasma membrane calcium pump: biochemistry, physiology and molecular pathology

  • Prof. Ernesto Carafoli
• 60 min
  29. The calcium saga: a matter of life and death

  • Prof. Pierluigi Nicotera
• 22 min
  30. Ca2+ efflux and Ca2+ signals

  • Dr. Anne Green
• 31 min
  31. Organellar calcium pumps of the golgi and ER: from yeast to human

  • Prof. Rajini Rao
• 58 min
  32. Spatial and temporal aspects of cellular calcium signals: 'elementary' signals and waves

  • Prof. Ian Parker
• 26 min
  33. Modeling Ca2+ signals

  • Dr. David Friel
• 42 min
  34. Regulation and role of mitochondrial Ca2+ homeostasis

  • Prof. Rosario Rizzuto
• 46 min
  35. Genetic defects and calcium

  • Prof. Tullio Pozzan
• 20 min
  36. Monitoring Ca2+ concentration in living cells

  • Dr. Marisa Brini
• 34 min
  37. Voltage-dependent calcium channels

  • Prof. Annette Dolphin
• 37 min
  38. Intracellular Ca2+ signaling: calcium influx

  • Prof. Anant Parekh
• 53 min
  39. The InsP3 receptor calcium release channel

  • Prof. J. Kevin Foskett
• 57 min
  40. cADPR and NAADP: messengers for calcium signalling

  • Prof. Antony Galione
• 43 min
  41. Ryanodine receptors and cardiac function

  • Prof. David Eisner
• 45 min
  42. Sodium-calcium exchange

  • Prof. John Reeves
• 34 min
  43. Ca2+ dynamics between mitochondria and the endoplasmic reticulum

  • Dr. Wolfgang Graier
• 26 min
  44. Nuclear calcium signaling

  • Dr. Oleg Gerasimenko
  • Dr. Julia Gerasimenko
• 41 min
  45. Calcium-regulated adenylyl cyclases and cyclic AMP compartmentalization

  • Prof. Dermot Cooper
• 43 min
  46. Polycystins, calcium signaling and pathogenesis of polycystic kidney disease

  • Prof. Laura del Senno
• 54 min
  47. Regulation of intracellular calcium signaling, localized signals and oscillations

  • Prof. Barbara Ehrlich
• 60 min
  48. Molecular identification of the CRAC channel

  • Prof. Michael Cahalan
• 38 min
  49. Pancreatitis and calcium signaling

  • Prof. Ole Petersen
• 47 min
  50. Mechanism-based therapies for heart failure and cardiac arrhythmias

  • Prof. Andrew Marks

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Outline
3. Overview
4. Ryanodine receptor - an overview
5. Molecular cloning of human RyR2
6. Complexity of RyR function
7. RyR domain architecture
8. RyR channel regulation
9. Intrinsic lattice formation of RyR tetramers
10. "Coupled gating": synchronous channel opening
11. Regulators of RyR channel activity
12. Channel regulation by divalent cations
13. Regulation by phosphorylation
14. Regulation by protein-protein interaction
15. Building a picture of RyR domain localization
16. Leucine zippers (LZ) and coiled-coil domains
17. Coiled-coil domains: stitching monomers together
18. Role of LZ motifs in RyR protein-protein interaction
19. Creation of cellular 'super-networks'
20. Disruption of the RyR macromolecular complex
21. A 'unifying' mechanism of pathological Ca leak
22. Genetic basis of RyR dysfunction
23. Mutations in EC coupling: arrhythmogenic culprits
24. The genetic basis of stress-induced VT
25. Novel mutations identified over the years
26. Ryanodine receptor mutations
27. Ryanodine receptor mutations clustering
28. Amino acid changes exhibit locus specificity
29. Effect of mutations loci on functional defects
30. The molecular basis of RyR-linked diseases
31. The basis of skeletal muscle pathologies
32. Molecular basis of malignant hyperthermia
33. The use of recombinant systems
34. Experimental systems: HL-1 cells
35. HL-1 cardiomyocytes: a functional syncitium
36. Monitoring intracellular expression
37. Visualising RyR2 in cardiomyocytes
38. Mechanistic defects in RyR2 mutants
39. Molecular aspects of RyR2-linked arrhythmia
40. Domain 'unzipping' and channel instability
41. Telling proteins apart...
42. Fluorescence resonance energy transfer (FRET)
43. Interacting (I-) domain: a molecular 'hinge'
44. The link between channel opening and Ca release
45. Summary

Topics Covered

• Molecular cloning of RyR
• The identification of functional domains
• RyR channel structure
• Channel regulation by: a) inter-RyR interaction, b) divalent cations and c) protein:protein interactions
• The role of leucine zippers in assembling the 'macromolecular complex'
• Defective protein:protein interactions in cardiac disease
• Genetic basis of RyR dysfunction
• Identification of mutations in cardiac RyR
• Functional characterization of RyR mutations
• Investigating interdomain interactions using state-of-the-art imaging tools
• Pathological defects in channel stability

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Therapeutic Areas:

• Cardiovascular & Metabolic

Talk Citation

Publication History

• Published on October 1, 2007
• Reviewed on May 9, 2022

Financial Disclosures

• Dr. Christopher George has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
• Prof. F. Anthony Lai has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.