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See webinar details- Introduction to Calcium Signaling
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72 min
1. Introduction to cellular calcium signaling- Dr. Martin Bootman
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20 min
2. Monitoring Ca2+ concentration in living cells- Dr. Marisa Brini
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30 min
3. Cell boundary theorem- Prof. Eduardo Ríos
- Calcium Influx
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19 min
4. Arachidonic acid and store-independent Ca2+ entry- Dr. Luca Munaron
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34 min
5. Voltage-dependent calcium channels- Prof. Annette Dolphin
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34 min
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37 min
7. Intracellular Ca2+ signaling: calcium influx- Prof. Anant Parekh
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60 min
8. Molecular identification of the CRAC channel- Prof. Michael Cahalan
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35 min
- Calcium Release
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53 min
10. The InsP3 receptor calcium release channel- Prof. J. Kevin Foskett
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31 min
11. Molecular biology of ryanodine receptors: an overview- Dr. Christopher George
- Prof. F. Anthony Lai
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57 min
12. cADPR and NAADP: messengers for calcium signalling- Prof. Antony Galione
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43 min
13. Ryanodine receptors and cardiac function- Prof. David Eisner
- Calcium Efflux and Sequestration
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45 min
14. Sodium-calcium exchange- Prof. John Reeves
- Organelle Calcium
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42 min
15. Regulation and role of mitochondrial Ca2+ homeostasis- Prof. Rosario Rizzuto
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54 min
16. Peroxisomes and Golgi apparatus as players in Ca2+ homeostasis- Dr. Paola Pizzo
- Dr. Alex Costa
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34 min
17. Ca2+ dynamics between mitochondria and the endoplasmic reticulum- Dr. Wolfgang Graier
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26 min
18. Nuclear calcium signaling- Dr. Oleg Gerasimenko
- Dr. Julia Gerasimenko
- Spatiotemporal Calcium Signals
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54 min
19. Regulation of intracellular calcium signaling, localized signals and oscillations- Prof. Barbara Ehrlich
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43 min
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41 min
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49 min
- Calcium Effectors
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34 min
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41 min
24. Calcium-regulated adenylyl cyclases and cyclic AMP compartmentalization- Prof. Dermot Cooper
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32 min
25. Calcium and transcription-coupling- Dr. Karen Lounsbury
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50 min
26. Cellular calcium (Ca2+) buffers- Prof. Dr. Beat Schwaller
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24 min
27. Extracellular calcium signaling- Dr. Aldebaran M. Hofer
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50 min
28. Ca2+, fertilization and egg activation- Prof. Karl Swann
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24 min
29. Calcium regulation of transcription in plants- Prof. Hillel Fromm
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34 min
30. Mechanisms regulating STIM expression and function in Ca2+ signaling- Dr. Jonathan Soboloff
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46 min
31. Dynamic signal encoding in the S. cerevisiae calcium response- Dr. Chiraj Dalal
- Calcium and Disease
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43 min
32. Polycystins, calcium signaling and pathogenesis of polycystic kidney disease- Prof. Laura del Senno
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28 min
33. Ca2+ alterations in familial Alzheimer's disease (FAD)- Dr. Paola Pizzo
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38 min
34. Pancreatitis and calcium signaling- Prof. Ole Petersen
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47 min
35. Mechanism-based therapies for heart failure and cardiac arrhythmias- Prof. Andrew Marks
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46 min
36. Genetic defects and calcium- Prof. Tullio Pozzan
- Archived Lectures *These may not cover the latest advances in the field
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31 min
37. Calcium, calmodulin and calcineurin- Prof. Stephen Bolsover
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34 min
38. Calcium flickers steer cell migration- Prof. Heping Cheng
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28 min
39. Automated Ca2+ imaging of chemosensory neurones in C.elegans- Dr. Nikos Chronis
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46 min
40. Ca2+ and the regulation of small GTPases- Prof. Peter Cullen
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35 min
41. Genetically encoded Ca2+ indicators: molecular scale measurements in mammals in vivo- Dr. Michael I. Kotlikoff
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35 min
42. Capacitative (store-operated) calcium entry- Dr. Jim Putney
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47 min
43. The molecular biology of the inositol trisphosphate receptor- Dr. Randen Patterson
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50 min
44. Coordinated Ca2+ release from intracellular Ca2+ stores- Prof. Ole Petersen
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45 min
45. The plasma membrane calcium pump: biochemistry, physiology and molecular pathology- Prof. Ernesto Carafoli
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60 min
46. The calcium saga: a matter of life and death- Prof. Pierluigi Nicotera
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22 min
47. Ca2+ efflux and Ca2+ signals- Dr. Anne Green
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31 min
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58 min
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26 min
50. Modeling Ca2+ signals- Dr. David Friel
Printable Handouts
Navigable Slide Index
- Introduction
- Outline
- Overview
- Ryanodine receptor - an overview
- Molecular cloning of human RyR2
- Complexity of RyR function
- RyR domain architecture
- RyR channel regulation
- Intrinsic lattice formation of RyR tetramers
- "Coupled gating": synchronous channel opening
- Regulators of RyR channel activity
- Channel regulation by divalent cations
- Regulation by phosphorylation
- Regulation by protein-protein interaction
- Building a picture of RyR domain localization
- Leucine zippers (LZ) and coiled-coil domains
- Coiled-coil domains: stitching monomers together
- Role of LZ motifs in RyR protein-protein interaction
- Creation of cellular 'super-networks'
- Disruption of the RyR macromolecular complex
- A 'unifying' mechanism of pathological Ca leak
- Genetic basis of RyR dysfunction
- Mutations in EC coupling: arrhythmogenic culprits
- The genetic basis of stress-induced VT
- Novel mutations identified over the years
- Ryanodine receptor mutations
- Ryanodine receptor mutations clustering
- Amino acid changes exhibit locus specificity
- Effect of mutations loci on functional defects
- The molecular basis of RyR-linked diseases
- The basis of skeletal muscle pathologies
- Molecular basis of malignant hyperthermia
- The use of recombinant systems
- Experimental systems: HL-1 cells
- HL-1 cardiomyocytes: a functional syncitium
- Monitoring intracellular expression
- Visualising RyR2 in cardiomyocytes
- Mechanistic defects in RyR2 mutants
- Molecular aspects of RyR2-linked arrhythmia
- Domain 'unzipping' and channel instability
- Telling proteins apart...
- Fluorescence resonance energy transfer (FRET)
- Interacting (I-) domain: a molecular 'hinge'
- The link between channel opening and Ca release
- Summary
Topics Covered
- Molecular cloning of RyR
- The identification of functional domains
- RyR channel structure
- Channel regulation by: a) inter-RyR interaction, b) divalent cations and c) protein:protein interactions
- The role of leucine zippers in assembling the 'macromolecular complex'
- Defective protein:protein interactions in cardiac disease
- Genetic basis of RyR dysfunction
- Identification of mutations in cardiac RyR
- Functional characterization of RyR mutations
- Investigating interdomain interactions using state-of-the-art imaging tools
- Pathological defects in channel stability
Links
Series:
Categories:
Therapeutic Areas:
Talk Citation
George, C. and Lai, F.A. (2022, May 9). Molecular biology of ryanodine receptors: an overview [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 26, 2024, from https://doi.org/10.69645/UMJR9240.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Christopher George has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
- Prof. F. Anthony Lai has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
