• Introduction to Calcium Signaling
• 72 min
  1. Introduction to cellular calcium signaling

  • Dr. Martin Bootman
• 20 min
  2. Monitoring Ca2+ concentration in living cells

  • Dr. Marisa Brini
• 30 min
  3. Cell boundary theorem

  • Prof. Eduardo Ríos
• Calcium Influx
• 19 min
  4. Arachidonic acid and store-independent Ca2+ entry

  • Dr. Luca Munaron
• 34 min
  5. Voltage-dependent calcium channels

  • Prof. Annette Dolphin
• 34 min
  6. Protein networks of voltage-gated Ca2+ channels – proteomic & functional analysis of local Ca2+ signalling

  • Prof. Bernd Fakler
• 37 min
  7. Intracellular Ca2+ signaling: calcium influx

  • Prof. Anant Parekh
• 60 min
  8. Molecular identification of the CRAC channel

  • Prof. Michael Cahalan
• 35 min
  9. TRP channels with diverse permeability profiles: regulation of blood pressure and fertility

  • Prof. Marc Freichel
• Calcium Release
• 53 min
  10. The InsP3 receptor calcium release channel

  • Prof. J. Kevin Foskett
• 31 min
  11. Molecular biology of ryanodine receptors: an overview

  • Dr. Christopher George
  • Prof. F. Anthony Lai
• 57 min
  12. cADPR and NAADP: messengers for calcium signalling

  • Prof. Antony Galione
• 43 min
  13. Ryanodine receptors and cardiac function

  • Prof. David Eisner
• Calcium Efflux and Sequestration
• 45 min
  14. Sodium-calcium exchange

  • Prof. John Reeves
• Organelle Calcium
• 42 min
  15. Regulation and role of mitochondrial Ca2+ homeostasis

  • Prof. Rosario Rizzuto
• 54 min
  16. Peroxisomes and Golgi apparatus as players in Ca2+ homeostasis

  • Dr. Paola Pizzo
  • Dr. Alex Costa
• 34 min
  17. Ca2+ dynamics between mitochondria and the endoplasmic reticulum

  • Dr. Wolfgang Graier
• 26 min
  18. Nuclear calcium signaling

  • Dr. Oleg Gerasimenko
  • Dr. Julia Gerasimenko
• Spatiotemporal Calcium Signals
• 54 min
  19. Regulation of intracellular calcium signaling, localized signals and oscillations

  • Prof. Barbara Ehrlich
• 43 min
  20. Modeling Ca2+ signals: understanding Ca2+ regulatory networks in cells: introduction and basic concepts

  • Dr. David Friel
• 41 min
  21. Modeling Ca2+ signals: understanding Ca2+ regulatory networks in cells - Ca2+ stores: endoplasmic reticulum, calcium oscillations

  • Dr. David Friel
• 49 min
  22. Modeling Ca2+ signals: understanding Ca2+ regulatory networks in cells - Ca2+ stores: mitochondria, models of spatially non-uniform Ca2+ signals, applications to analysis of experimental results

  • Dr. David Friel
• Calcium Effectors
• 34 min
  23. Neuronal calcium sensor proteins: calcium sensors and regulators of diverse cellular functions

  • Prof. Bob Burgoyne
• 41 min
  24. Calcium-regulated adenylyl cyclases and cyclic AMP compartmentalization

  • Prof. Dermot Cooper
• 32 min
  25. Calcium and transcription-coupling

  • Dr. Karen Lounsbury
• 50 min
  26. Cellular calcium (Ca2+) buffers

  • Prof. Dr. Beat Schwaller
• 24 min
  27. Extracellular calcium signaling

  • Dr. Aldebaran M. Hofer
• 50 min
  28. Ca2+, fertilization and egg activation

  • Prof. Karl Swann
• 24 min
  29. Calcium regulation of transcription in plants

  • Prof. Hillel Fromm
• 34 min
  30. Mechanisms regulating STIM expression and function in Ca2+ signaling

  • Dr. Jonathan Soboloff
• 46 min
  31. Dynamic signal encoding in the S. cerevisiae calcium response

  • Dr. Chiraj Dalal
• Calcium and Disease
• 43 min
  32. Polycystins, calcium signaling and pathogenesis of polycystic kidney disease

  • Prof. Laura del Senno
• 28 min
  33. Ca2+ alterations in familial Alzheimer's disease (FAD)

  • Dr. Paola Pizzo
• 38 min
  34. Pancreatitis and calcium signaling

  • Prof. Ole Petersen
• 47 min
  35. Mechanism-based therapies for heart failure and cardiac arrhythmias

  • Prof. Andrew Marks
• 46 min
  36. Genetic defects and calcium

  • Prof. Tullio Pozzan
• Archived Lectures *These may not cover the latest advances in the field
• 31 min
  37. Calcium, calmodulin and calcineurin

  • Prof. Stephen Bolsover
• 34 min
  38. Calcium flickers steer cell migration

  • Prof. Heping Cheng
• 28 min
  39. Automated Ca2+ imaging of chemosensory neurones in C.elegans

  • Dr. Nikos Chronis
• 46 min
  40. Ca2+ and the regulation of small GTPases

  • Prof. Peter Cullen
• 35 min
  41. Genetically encoded Ca2+ indicators: molecular scale measurements in mammals in vivo

  • Dr. Michael I. Kotlikoff
• 35 min
  42. Capacitative (store-operated) calcium entry

  • Dr. Jim Putney
• 47 min
  43. The molecular biology of the inositol trisphosphate receptor

  • Dr. Randen Patterson
• 50 min
  44. Coordinated Ca2+ release from intracellular Ca2+ stores

  • Prof. Ole Petersen
• 45 min
  45. The plasma membrane calcium pump: biochemistry, physiology and molecular pathology

  • Prof. Ernesto Carafoli
• 60 min
  46. The calcium saga: a matter of life and death

  • Prof. Pierluigi Nicotera
• 22 min
  47. Ca2+ efflux and Ca2+ signals

  • Dr. Anne Green
• 31 min
  48. Organellar calcium pumps of the golgi and ER: from yeast to human

  • Prof. Rajini Rao
• 58 min
  49. Spatial and temporal aspects of cellular calcium signals: 'elementary' signals and waves

  • Prof. Ian Parker
• 26 min
  50. Modeling Ca2+ signals

  • Dr. David Friel

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Molecular identification of the CRAC channel
3. Outline
4. T cells in the lymph node
5. T cell - dendritic cell interactions in lymph node
6. T-Cell / APC interaction dynamics
7. Ca and gene expression in T cells
8. How does calcium enter the cell
9. Ion channels in T lymphocytes
10. Ca signaling in T lymphocytes
11. CRAC channel and lymphocyte activation
12. Genes encoding channel components
13. CRAC current in Drosophila S2 cells
14. Screening for CRAC in Drosophila S2 cells
15. Suppression of Ca influx by Stim dsRNA
16. Patch clamp recordings confirm STIM expression
17. Suppression of CRAC current by Stim dsRNA
18. STIM1 suppression in Jurkat T cells
19. Effects of Stim (STIM1) supression
20. Two functions of Stim
21. Stim sequence and domains
22. Overexpression of STIM and EF hand mutants
23. Activation of CRAC channels in S2 cells
24. Overexpression of wild-type STIM1 in the ER
25. Re-distribution of STIM1 following store depletion
26. Hotspots of STIM1 following store depletion
27. Subcellular distribution of STIM1: conclusion
28. Dual functions of Stim
29. Genome-wide screen for CRAC
30. Genome-wide RNAi screen for SOC influx
31. Quantitative screening results
32. Top 10 Hits
33. RNAi knock-down of olf186-F gene
34. olf186-F conserved gene family
35. Proposed olf186-F membrane topology
36. Overexpression of olf186-F and olf186-F + Stim
37. Accelerating CRAC current development
38. Functional expression olf186-F + STIM as SOC
39. Giant CRAC currents induced by olf186-F + Stim
40. Amplifying CRAC current
41. Molecular identity of the CRAC channel
42. Mutants of olf186-F co-expressed with Stim
43. Mutation E180D Orai alters I-V shape
44. CRAC current upon choline substitution for Na
45. E180D Orai alters divalent cation selectivity
46. Calcium block of CRAC current in E180D Orai
47. Altered monovalent ion selectivity of E180D Orai
48. More alterations in E180D Orai
49. Charge-neutralizing Orai mutations in S1-S2
50. pA/pF of WT and mutants in S1-S2
51. Gadolinium block in WT and mutants
52. olf186-F is a bona fide ion channel
53. CRAC = olf186-F (Orai, CRACM)
54. How does the CRAC channel open?
55. Stim / olf186-F co-immunoprecipitation
56. Syntaxin 5 (and tsr) knockdown
57. SERCA knockdown
58. CRAC channel activation pathway
59. Several possibilities for activation
60. CRAC summary
61. Acknowledgments (1)
62. Acknowledgments (2)

Topics Covered

• Background on Calcium signaling and ion channels in the immune system, role of CRAC channels in lymphocyte activation
• Discovery of Stim and STIM1 by RNA interference screen in Drosophila S2 cells
• Stim as an ER Calcium sensor and as a messenger to the plasma membrane
• Genome-wide RNA interference screen and the discovery of Orai
• Amplifying CRAC current by co-expression of Stim and Orai
• Molecular identification of CRAC channels by altered ion selectivity in a mutant of Orai
• How does the CRAC channel activate?

Links

Series:

• Calcium Signaling I

Categories:

• Cell Biology
• Immunology

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Michael Cahalan has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.