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Printable Handouts
Navigable Slide Index
- Introduction
- Genetic modification (GM)
- A. tumefaciens and crown gall disease
- GM of potato with A. tumefaciens (1)
- GM of potato with A. tumefaciens (2)
- GM of potato with A. tumefaciens (3)
- GM of potato with A. tumefaciens (4)
- GM of wheat by particle bombardment
- GM modification of wheat
- Why is GM used in modern plant breeding? (1)
- Why is GM used in modern plant breeding? (2)
- Genome (gene) editing
- Genome editing: A suite of technologies
- Genome editing with oligonucleotides
- Genome editing using targeted nucleases
- TALENs and ZFNs
- CRISPR-Cas9
- GM crop areas in 26 countries, 2016
- Global area of biotech crops, 2017
- Global adoption rates for biotech crops, 2017
- Global area of biotech crops by trait, 2017
- Input (1st generation) traits
- Gluphosinate and bromoxynil tolerance
- Herbicide-tolerant GM crops
- Genome editing for herbicide tolerance
- Bacillus thuringiensis
- Insect-resistant GM crops
- Adoption of Bt cotton in India, 2002-2009
- Indian cotton production, 1991-2013 (1)
- Indian cotton production, 1991-2013 (2)
- Bt corn in Europe
- Area of Bt maize grown in Europe, 2006-2016
- Maize yield in Italy, France and Spain, 1994-2015
Topics Covered
- Genetic modification (GM) of plants
- Techniques used to produce GM plants
- Agrobacterium tumefaciens
- Genetic modification of wheat
- Genome editing with oligonucleotides
- Genome editing with targeted nucleases
- TALENS, ZFNs and CRISPR-Cas9
- New breeding technologies using genome editing
- Use of GM plants in agriculture
- 1st generation traits in GM crops
Talk Citation
Halford, N.G. (2019, August 29). Crop biotechnology update 1: genome editing technologies [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/IAMO7253.Export Citation (RIS)
Publication History
Financial Disclosures
- Nigel Halford is funded at Rothamsted Research by the Biotechnology and Biological Sciences Research Council (BBSRC) of the United Kingdom through the Designing Future Wheat Programme (BB/P016855/1). He is the project leader for a BBSRC SWBio iCASE Doctoral Training Partnership Studentship ‘Genome editing for low acrylamide wheat’, which involves the following partners: Agriculture and Horticulture Development Board; KWS UK Ltd; Saaten Union UK Ltd; RAGT Seeds Ltd; Syngenta UK Ltd; Limagrain UK Ltd. University of Bristol. He is also the project leader for a BBSRC Collaborative Training Partnership studentship ‘Crop management strategies for low asparagine grains to limit acrylamide-forming potential’, with partners Mondelēz International and University of Reading. The views expressed in the presentation do not necessarily reflect those of these funders and partners, who had no role in its preparation.
Crop biotechnology update 1: genome editing technologies
Published on August 29, 2019
32 min
A selection of talks on Methods
Transcript
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0:00
Hello, my name is Nigel Halford.
I'm a senior scientist at Rothamsted Research in the UK.
I am going to talk about "Crop Biotechnology" and
give you an update on the current situation.
0:13
So genetic modification or GM,
not a new technology anymore.
It's a technology used for crop improvement since the 1980s.
It allows the introduction of
a single and specific gene or a small number of genes into a plant,
that's something you can't do by other methods in plant breeding.
So it's not replacing other methods in plant breeding,
it's an additional tool that plant breeders can use.
It's achieved, I'm going to show you a couple of methods.
One, you hitch a lift on a DNA fragment from nature's genetic engineer,
that's a soil bacterium called Agrobacterium tumefaciens,
or second method is by bombarding plant tissue with tiny gold particles coated with DNA.
0:53
So Agrobacterium tumefaciens is a naturally-occurring soil bacterium,
and the bacterium has been genetically engineering or genetically
modifying plants long before humans discovered how to do it.
It causes crown gall disease in plants.
What it does, it infects wounded plant tissue and it
inserts a piece of it's own DNA into the DNA of the plant cell,
and that does a number of things.
It has information on that DNA to cause a plant cell to
proliferate and you get one of these masses of undifferentiated cells,
you can see on this slide,
they can get as large as a football on a tree and that's a picture
I took in Ashridge Forest in Hertfordshire a few years ago.
It also has information in that piece of DNA to cause the cells within
that mass to stop producing unusual compounds on which the bacterium feeds.
So it genetically modifies the plant cells to feed itself.
So we can piggyback on that system.
So we take out some of the genes that
the bacterium would want to put into the plant cell.
We can put 1, 2,
3 genes that we would like to go into the plant cell.