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1. Introduction
2. Importance of measuring Ca2+ concentrations
3. Ca signaling and function in different cells
4. Controlling Ca homeostasis
5. Luminescent and fluorescent Ca probes
6. Synthetic probes
7. Fura-2/AM as a fluorescent Ca indicator dye
8. The detection system
9. Ca chelators derivatives: non-ratiometric probes
10. Ca chelators derivatives: ratiometric probes
11. Ratio (intensity) versus concentration
12. Targeted Ca probes
13. Measuring mitochondrial [Ca] with rhod-2
14. Measuring organelle calcium
15. Aequorin: a gene encoded Ca probe
16. Recombinant probes
17. Advantages of aequorin
18. Aequorin activity as an indicator
19. Reconstituting aequorin (protein+prosthetic group)
20. Reconstituting aequorin where [Ca] is high
21. Aequorin probes
22. Using aequorin in population studies
23. Measurement of [Ca] in the cytosole
24. Measurement of [Ca] in the ER lumen
25. Measurement of [Ca] in the mitochondria
26. Disadvantages of aequorin (1)
27. Disadvantages of aequorin (2)
28. GFP-based probes
29. Recombinant GFP-based fluorescent probes
30. FRET:fluorescence resonance energy transfer
31. Yellow cameleon
32. Enhanced mitochondrial targeting of protein probes
33. Camgaroo probe
34. Ratiometric pericam probe
35. New Ca sensors

Topics Covered

• Introduction to Calcium homeostasis
• Synthetic fluorescent Calcium probes
• Ratiometric and non-ratiometric probes
• Calibration
• Recombinant probes: the targeted probes
• Aequorin
• Targeting strategies
• Wide dynamic range
• Applications: some examples
• GFP-based probes
• Advantages and disadvantages
• Imaging
• FRET based probes

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Dr. Marisa Brini has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.