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1. Introduction
2. Aims
3. Intracellular proteases
4. The 20S 'core' proteasome
5. Properties of the 20S 'core' proteasome
6. Proteasomal particles
7. The proteasome-ubiquitin system
8. The proteasomal system
9. Hypothesis: protein oxidation and proteasome
10. Degradation of oxidized proteins: 'in vitro' studies
11. Degradation of hydrogen peroxide-oxidized ferritin
12. Degradation of ferritin oxidized by other oxidants
13. Oxidation, degradation and protein function: ferittin
14. Oxidation and protein function: GAPDH
15. Degradation of oxidized GAPDH
16. Degradation of oxidized proteins in vitro - summary
17. Protein surface hydrophobicity
18. Degradation of oxidized RNase A
19. FT-IR spectra of RNase A
20. Changes in RNase due to oxidation
21. Unfolding of oxidized proteins
22. Stability of the 20S proteasome towards oxidation
23. Degradation by the proteasome in vitro - summary
24. Degradation of oxidized proteins: mammalian cells
25. Oxidative stress and protein turnover
26. Proteasome-dependent increased proteolysis
27. Stability towards oxidation: 26S vs. 20S form
28. Loss of E1 and ubiquitination in E36ts20 cells
29. Degradation of oxidized proteins in E1 mutants
30. Degradation of oxidized proteins in cells: summary
31. What happens if degradation fails ?
32. Protein aggregates inhibit the proteasome
33. Particle uptake by fibroblasts
34. Proteolytic activity during lipofuscin treatment
35. Proteasome activity in Alzheimer disease brain
36. PHFs bind to the proteasome
37. Summary
38. Acknowledgment

Topics Covered

• Oxidized proteins
• Intracellular proteases
• The 20S core proteasome
• Proteasomal particles
• The proteasome-ubiquitin system
• Protein oxidation and proteasome
• Degradation of oxidized proteins and protein function
• Protein surface hydrophobicity
• Unfolding of oxidized proteins
• Stability of the 20S proteasome towards oxidation
• Oxidative stress and protein turnover
• Oxidation-induced proteolysis
• Degradation of oxidized proteins in cells
• What happens if degradation fails?
• Protein aggregates inhibit the proteasome
• Particle uptake by fibroblasts

Links

Series:

• Free Radicals and the Oxygen Paradox

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Grune, T. (2007, November 1). Protein oxidation and removal of oxidized proteins by proteolysis [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/HAON8111.
Export Citation (RIS)

Publication History

• Published on November 1, 2007
• Archived on May 31, 2018

Financial Disclosures

• Prof. Tilman Grune has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.