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1. Introduction
2. Formation of reactive oxygen species
3. Metal ion-catalyzed Haber-Weiss reaction
4. Sites of oxidative damage in DNA
5. Types of DNA damage by free radicals
6. Reactions of hydroxyl radical with purines
7. Formation of purine products
8. Formation of 8,5'-cyclopurine-2'-deoxynucleosides
9. Products of oxidative damage to DNA bases
10. Measurement of DNA damage and repair
11. Biological responses to genomic DNA damage
12. Pathways for processing of a DNA lesion in cells
13. Mutagenesis by oxidative DNA damage products
14. Mechanisms of base-excision repair
15. Activity of a DNA glycosylase
16. Repair of 8,5'-cyclopurine-2'-deoxynucleosides
17. DNA glycosylases in base-excision repair
18. Substrate specificities of DNA glycosylases (1)
19. Substrate specificities of DNA glycosylases (2)
20. Determination of excision by a DNA glycosylase
21. Basic equations of enzyme kinetics
22. Substrates of E. coli Fpg
23. Substrates of eukaryotic OGG1 proteins
24. Excision by polymorphic variants of human OGG1
25. Substrates of E. coli endonuclease III (Nth)
26. Substrates of E. coli Nei
27. Substrates of DNA glycosylases - summary
28. In vivo assays of NTH1 and OGG1activity (1)
29. In vivo assays of NTH1 and OGG1activity (2)
30. DNA lesions in DNA of NTH1 and OGG1 KO mice
31. Role of NEIL1 in disease
32. Mouse and human NEIL1 proteins
33. Substrates of human and mouse NEIL1 proteins
34. Conservation of human NEIL1
35. Activities of human NEIL1 variants
36. Excision by polymorphic variants of human NEIL1
37. Frequency of polymorphic variants of human NEIL1
38. DNA lesions in organs of neil1 knockout mice
39. Metabolic syndrome phenotype in neil1 KO mice
40. Cockayne syndrome
41. CSB structure and location of designed mutants
42. Activity of extracts of wt and CSB-mutant cells
43. 8-hydroxy-2'-deoxyadenosine in CSB mutant cells
44. DNA lesions in fibroblasts from CSB patients
45. Collaborators

Topics Covered

• Formation of reactive oxygen species
• Oxidative damage to DNA bases by free radicals
• Techniques used for the measurement of DNA damage and repair
• Biological responses to genomic DNA damage
• Mechanisms of base-excision repair
• Determination of substrate specificities of DNA glycosylases in-vitro and in-vivo
• Mouse and human NEIL1 proteins
• Cockayne syndrome
• CSB protein

Links

Series:

• Free Radicals and the Oxygen Paradox

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Dizdaroglu, M. (2007, November 1). Oxidative DNA damage: mechanisms, repair and disease [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/RBBK4968.
Export Citation (RIS)

Publication History

• Published on November 1, 2007
• Archived on December 10, 2018

Financial Disclosures

• Prof. Miral Dizdaroglu has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.