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Navigable Slide Index

1. Introduction
2. Talk outline
3. Protein targeting
4. Protein targeting and secretion in E. coli
5. SRP-mediated targeting
6. Conservation of the SRP/SR
7. Cryo-EM structure of ribosome-SRP complex
8. Co-translational targeting by the SRP
9. Specific aspects of E. coli SRP
10. Trigger factor
11. SecYEG
12. Crystal structure of the Sec-translocon
13. Biogenesis of two model E. coli IMPs
14. The model proteins
15. In vitro translation and cross-linking
16. Studying FtsQ and Lep
17. Cross-linking 108FtsQ
18. A dual role of YidC in IMP integration
19. Properties of YidC
20. Interactions in FtsQ membrane integration (1)
21. Interactions in FtsQ membrane integration (2)
22. Membrane integration of Lep H1
23. Two models of membrane integration
24. Rapid movement of H1 into lipid bilayer
25. Membrane integration of Lep H2
26. Membrane integration of Lep - summary
27. Substrate specificity of YidC
28. YidC depletion affects F1F0 ATPase biogenesis
29. YidC depletion affects Cyo oxidase biogenesis
30. Properties of F0c and CyoA
31. Membrane assembly of F0c
32. Membrane assembly of CyoA
33. E. coli membrane proteins biogenesis
34. YidC: molecular chaperone and insertase?
35. X-ray structure of SecYEG
36. People and projects
37. Acknowledgements

Topics Covered

• SRP-mediated targeting to the inner membrane
• Structure and function of the Sec-translocon in membrane insertion
• Role of YidC as an insertase and membrane chaperone

Links

Series:

• Molecular Chaperones

Categories:

• Biochemistry
• Cell Biology
• Microbiology

Talk Citation

Luirink, J. (2007, October 1). The biogenesis of E. coli inner membrane proteins [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/COIS9876.
Export Citation (RIS)

Publication History

• Published on October 1, 2007
• Archived on August 13, 2020

Financial Disclosures

• Dr. Joen Luirink has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.