Tracking vector insertion sites to explore the biology of transduced cells in vivo

Published on September 3, 2014   35 min

Other Talks in the Series: Gene Transfer and Gene Therapy

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My name is Christof von Kalle from the National Center for Tumor Diseases in Heidelberg in the German Cancer Research Center. I'm going to be talking about tracking vector insertion sites to explore the biology of transduced cells in vivo.
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Integrating vectors have been used for a number of years for the genetic modification of cells. This has been particularly done extensively in that formation, hematopoiesis. Integrating vectors have the properties to place a copy of the profile DNA somewhere in the genome of the transfused sell. This integration occurs depending on the vector system in a semi-random fashion and is stable. You can see that a marking that occurs in a progenitor or a stem cell is actually then passed on to the progeny of that cell, meaning that sampling of the peripheral blood and analysis for the integration sites can delineate the activity of progenitor and stem cells in terms of their contribution to different blood lineages, the numbers or cells, and their activity over time.
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Tracking vector insertion sites to explore the biology of transduced cells in vivo

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