Hello, this is Adam Katz.
And the title of my presentation is
"Stem Cells from Adipose Tissue."
I am an associate
professor in the division
of plastic surgery at the
University of Florida.
As part of full disclosure, I would
like to mention that I am a named
inventor on issued and or
pending patents related
to the adipose field, including
adipose-derived cells and matrix.
And I have in the past received
royalties related to this IP.
I also serve on the
board of directors
or have been a founder
on at least two companies
that operate in this field.
As a brief overview
for my presentation,
I will be giving a brief background
on the adipose-derived cell,
as well as some of the
past and current science
related to these cells.
We'll also discuss briefly some of
the regulatory issues surrounding
the translation of
and then the translation and
commercialization of these cells
To begin, let's discuss adipose
tissue, which is a tissue many
of us are well aware
of and have learned
about in medical school or
other stages of training.
Many of us were taught that
the function of adipose tissue
was simply for storing
energy and providing
padding to important structures.
There are two main physiological
subcompartments of adipose tissue.
One is the parenchyma, which is
the functional part of the tissue.
In this case the adipocyte,
which stores energy.
And surrounding this is the stroma,
or the connective or supportive
structural framework of a tissue.
In this case, lots of fibroblasts
and endothelial vascular
We now know that adipose
tissue in extremely
complex and dynamic endocrine organ.
And from our standpoint, for
the purpose of this talk,
we'll be talking about the
regenerative potential of cells
and factors that are
derived from this tissue.
As such, adipose tissue
can now be viewed
as a tremendous biological
resource, in that it
is extremely abundant
in most individuals.
And it's very easy and safe to
harvest large amounts of tissue
through very small
incisions using traditional
and standard liposuction techniques.
This is very appealing to
patients, and it essentially
yields no donor side morbidity.
And in fact, to many
it is a highly attractive prospect
to donate their adipose tissue.
For these many
reasons, adipose tissue
is an extremely practical source of
biological resources that may prove
very useful in the emerging
regenerative medicine field.
The process to isolate
cells from adipose tissue
has been well described
in the '50s and '60s.
It essentially consists of the
steps listed here on slide six.
First, the tissue is
harvested, in our case
usually by liposuction procedure.
The tissue fragments then
are washed and concentrated
to get rid of any other fluids or
blood components as best possible.
The tissue is then subjected
to an enzymatic dissociation,
usually in the range
of 30 to 60 minutes.
And through various sequence of
centrifugation and/or filtration
steps, the free oil on in
adipocytes, which are buoyant,
will rise to the top
And the pelleted cells that
will pulled to the bottom, known
as the SVF, which stands for
stromal vascular fraction.
When these cells are plated
onto tissue culture plastic,
there is essentially a
cell enrichment step, which
is enrichment by adherence,
and this yields ASCs.
To further clarify the difference of
these cells, it's important for us
to make sure we are on
the same wave length
when talking about SVF and ASCs.
As mentioned, SVF stands for
stromal vascular fraction.
These are pelleted cells that
result after the initial enzymatic
digestion and centrifugation
and or filtration steps.
These cells have not been plated
into plastic or any other surface.
And these represent a mixed, or
heterogeneous, population of cells
that have been
described decades ago.
The SVF includes white blood cells,
including macrophages, neutrophils,
and lymphocytes, as well
as endothelial cells
and endothelial progenitor cells,
pericytes and other perivascular
type cells, stromal cells
such as fibroblasts,
and of course putative
progenitor and stem cells.