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1. Introduction
2. Outline
3. Clinical proteomics application
4. Study design: clinical aspect
5. Bacterial over-growth in urine affects proteomics
6. Sample size and statistical analysis matters!
7. European kidney and urine proteomics (EuroKUP)
8. EuroKUP four working groups
9. Initiatives
10. Scientific reporting of proteomic biomarker
11. Biomarker characterization
12. Clinical proteomics approaches
13. Bladder cancer statistics
14. Bladder cancer pathology
15. Current diagnostic tools for bladder cancer
16. Biomarker needs for bladder cancer
17. Identifying aggressive bladder cancer biomarkers
18. Why urine?
19. Identification of urine proteome
20. IMAC fractionation workflow for urinary proteins
21. Bladder cancer biomarkers by IMAC fractionation
22. Proteins identified in bladder cancer samples
23. Confirmation of expression differences by WB
24. TMA for profilin 1
25. Profilin expression and disease outcome
26. Profilin blockage
27. Cell line models for bladder cancer (T24M)
28. Development of a cell line model
29. T24M increased tumorigenic properties in vivo
30. Secretome analysis for protein expression
31. Protein expression in secretome: T24 vs. TS24M
32. Western blot and activity assay
33. SPARC Ab decreases T24M motility in vitro
34. Summary
35. Promising bladder cancer biomarkers
36. Clinical impact in bladder cancer management
37. From biomarker discovery to implementation
38. Guidelines for biomarker discovery
39. Getting closer to implementation
40. Roadmap to clinical implementation
41. Biomarker discovery: hypothesis driven
42. Multi-disciplinary collaborations
43. Acknowledgements
44. Funding sources
45. Thank you

Topics Covered

• Clinical proteomics: principles of experimental design and data reporting
• Clinical needs in bladder cancer
• Bladder Cancer Biomarker discovery in urine using proteomics approaches
• How to progress towards implementation

Links

Series:

• Clinical Proteomics

Categories:

• Biochemistry
• Cancer
• Cell Biology
• Clinical Practice
• Methods

Therapeutic Areas:

• Oncology

Talk Citation

Vlahou, A. (2013, November 5). Clinical proteomics in the investigation of bladder cancer [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/SWTL8916.
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Publication History

• Published on November 5, 2013

Financial Disclosures

• Prof. Antonia Vlahou has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.