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1. Introduction
2. What C. elegans are
3. Neuronal networks - from stimulation to behavior
4. Monitoring neuronal activity - calcium imaging
5. In vivo Ca imaging of chemosensory neurons
6. Automating In vivo Ca imaging
7. An automated calcium imaging platform
8. In vivo Ca imaging using a microfluidic chip
9. Processing single worms in the microfluidic chip
10. Fabrication of the microfluidic chip
11. The automated, in vivo calcium imaging platform
12. Automated single worm processing on-chip
13. Automated calcium imaging on-chip
14. A typical Ca response (chemosensory neuron)
15. Automated calcium imaging - possibilities
16. Effects of age on the function of sensory neurons
17. In vivo calcium imaging - parameters
18. Age effect on ASH neuronal response
19. Peak and slope of the ON response
20. Initial intracellular Ca level may be age-dependent
21. Calcium pathway in ASH neuron
22. Using in vivo imaging for drug screening
23. Approach - drug-treated vs untreated worms
24. Mianserin - yes, Resveratrol - no
25. Summary
26. Behind the scene

Topics Covered

• What C. elegans are
• Neuronal networks: from stimulation to behavior
• Monitoring neuronal activity
• Automating In vivo Ca imaging
• An automated calcium imaging platform
• A microfluidic chip
• Processing single worms
• Fabrication
• Automated single worm processing on-chip
• Automated calcium imaging on-chip
• A typical Ca response (chemosensory neuron)
• Effects of age on the function of sensory neurons
• Parameters
• Initial intracellular Ca level may be age-dependent
• Calcium pathway in ASH neuron
• Using in vivo imaging for drug screening
• Drug-treated vs. untreated worms

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology
• Neuroscience

Talk Citation

Chronis, N. (2012, March 18). Automated Ca2+ imaging of chemosensory neurones in C.elegans [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 26, 2024, from https://doi.org/10.69645/AVMV1687.
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Publication History

• Published on March 18, 2012
• Archived on August 13, 2020

Financial Disclosures

• Dr. Nikos Chronis has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.