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1. Introduction
2. Principles of mitochondrial protein homeostasis
3. Enzymatic mediators of protein homeostasis
4. Protein quality control in the matrix compartment
5. Question and approaches
6. In organello degradation assay
7. The mitochondrial protease Pim1
8. Degradation rates of imported reporter proteins
9. Folding state effects mitochondrial proteolysis
10. Biochemical properties of the protease Pim1
11. Substrate selectivity of the protease Pim1
12. 2D gel of mitochondrial proteins
13. Proteomic analysis of the protein turnover
14. Quantitative analysis of protein spot pattern
15. Proteolysis in organello
16. Pim1 substrates
17. Degradation in Fe/S cluster assembly mutants
18. Protein dynamics under oxidative stress
19. Protein degradation in ROS stressed mitochondria
20. Quantitative changes in mitochondrial proteome
21. Proteins with Fe/S cluster cofactors
22. In organello degradation of imported Ilv3
23. ROS-protective enzymes
24. Prx1 (peroxiredoxin)
25. Effect of oxidative stress on protein aggregation
26. Mitochondrial proteins affected by aggregation
27. Enzyme activities of affected proteins
28. Energy dependence of protein aggregation
29. Mitochondrial chaperone system protective effects
30. Pim1/LON protease protects against aggregation
31. Chaperones of the Hsp100/ClpB family
32. Reversal of protein aggregation
33. Resistance to oxidative stress in vivo
34. The mitochondrial protein quality control reaction
35. Acknowledgements

Topics Covered

• Protein homeostasis in mitochondria: the role of chaperones and proteases
• Principles of mitochondrial protein homeostasis
• Protein quality control in the matrix compartment
• Substrate selectivity of the mitochondrial protease Pim1
• Proteomic analysis of mitochondrial protein turnover
• Proteome alterations under oxidative stress
• Identification of aggregation-prone polypeptides
• Protective effects of chaperones
• Cooperation of Hsp78 and Pim1 in removal of aggregated polypeptides

Links

Series:

• Protein Homeostasis

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Voos, W. (2012, February 2). Protein homeostasis in mitochondria: chaperones and proteases of the mitochondrial matrix [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/LXQR8310.
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Publication History

• Published on February 2, 2012

Financial Disclosures

• Prof. Wolfgang Voos has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.