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1. Introduction
2. Objectives
3. Role of quality control in cell processes
4. Eukaryotic cells are highly compartmentalized
5. The ER environment
6. ER protein folding and quality control
7. Protein folding: ribonuclease A
8. Molecular chaperones
9. Protein disulfide isomerase (PDI)
10. PDIs which are present in the lumen
11. Prolyl cis-trans isomerase (PPI)
12. Molecular chaperones and foldases in the ER
13. N-glycosylation
14. N-glycan precursor synthesis
15. Glc3Man9GlcNAc2 glycan trimming in the ER
16. Calnexin: a lectin type chaperone
17. Calnexin transiently interacts with glycoproteins
18. The calnexin cycle (1)
19. Calnexin binds monoglucosylated glycoproteins
20. Binding of Glc1Man9 RNaseB intermediates
21. Calnexin lumenal domain
22. The calnexin cycle (2)
23. ERp57
24. RNase B refolding, catalyzed by ERp57 or PDI (1)
25. RNase B refolding, catalyzed by ERp57 or PDI (2)
26. Mapping the ERp57 binding site on calnexin
27. ERp57 structure and the calnexin binding site
28. PPIs also interact with calnexin/calreticulin
29. PDI-bb' domains can bind hydrophobic peptides
30. UDP-glucose glucosyl glycoprotein transferase
31. Man9GlcNAc2 glycoproteins from yeast DT111
32. Glucosylation of Man9GlcNAc2 glycopeptides
33. Rank order of peptides glucosylated by UGGT
34. Structure of beta-glucanase
35. UGGT substrates can be mutant but active
36. The calnexin cycle (3)
37. Disposal of misfolded glycoproteins by ERAD
38. The fate of glycoproteins
39. The ER quality control apparatus
40. ER quality control
41. Concluding words

Topics Covered

• The endoplasmic reticulum
• Protein folding
• Molecular chaperones
• N-glycosylation
• Lectin-type chaperones calnexin and calreticulin
• Link between protein folding and glycosylation
• ER quality control

Links

Series:

• Protein Homeostasis

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Thomas, D.Y. (2012, February 2). The recognition of misfolded glycoproteins in the endoplasmic reticulum [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/WLUG6188.
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Publication History

• Published on February 2, 2012

Financial Disclosures

• Dr. David Y. Thomas has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.