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1. Introduction
2. Concept of a molecular chaperone
3. Saccharomyces cerevisiae
4. TAP tag pull-downs (C-TAP)
5. Mapping chaperon interaction network in yeast
6. General principles: protein chaperon interaction
7. Top MIPS complexes interacting with chaperons
8. Top Pfam domains interacting with chaperons
9. Chaperon-chaperon interactions
10. Interactor overlap between two chaperones
11. Single vs. multiple pathways model (1)
12. Single vs. multiple pathways model (2)
13. New cellular pathways modulated by chaperones
14. Hsp90 and the conserved R2TP complex
15. Hsp90: overview
16. Integrated proteomics approaches
17. 2H Two-hybrid screen using an ordered array
18. TA tag pull-downs (forward and reverse)
19. SGA Synthetic lethal screen
20. GS Geldanamycin screen
21. Hsp90 putatively interacts with 627 proteins
22. Venn diagram
23. Hsp90 putatively interaction (1)
24. Hsp90 putatively interaction (2)
25. Origin of synthetically lethal proteins with HSP90
26. Proteomic data verification
27. Biochemistry of individual proteins/systems
28. Interesting ORF characteristics
29. Tah1 and Pih1
30. NMR structure of Tah1-MEEVD
31. Hsp90-Tah1-Pih1 form a ternary complex
32. Hsp90-Tah1-Pih1 complex function
33. Hsp90 maintains the stability of Pih1
34. Hsp90-Tah1 stabilize Pih1 in vivo (1)
35. Hsp90-Tah1 stabilize Pih1 in vivo (2)
36. Tah1 acts as a cofactor for HSP90
37. Tah1 & Pih1 target HSP90 to specific proteins
38. What is the function of the R2TP complex?
39. R2P role in the cell
40. Nop58 genetically interacts with R2TP
41. The simplified view
42. Box C/D snoRNP assembly
43. rRNA and snoRNA accumulation (1)
44. rRNA and snoRNA accumulation (2)
45. Proposed model
46. Summary
47. Funding
48. Thank you

Topics Covered

• Large scale proteomic screening in yeast
• The TAP-tag pulldown approach
• Understanding the principles governing protein homeostasis
• Mapping the network of the Hsp90 chaperone using physical and genetic interaction screens
• Unraveling the role of Hsp90 in pre-rRNA processing

Links

Series:

• Protein Homeostasis

Categories:

• Biochemistry
• Cell Biology
• Microbiology

Talk Citation

Houry, W.A. (2012, February 2). Mapping the molecular chaperone interaction network in yeast [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 3, 2024, from https://doi.org/10.69645/NNSB2557.
Export Citation (RIS)

Publication History

• Published on February 2, 2012

Financial Disclosures

• Prof. Walid A. Houry has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.