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Printable Handouts
Navigable Slide Index
- Introduction
- Outline
- Mast cells
- Mast cells in health and disease
- Mature human mast cell morphology
- Mast cell activation
- Intracellular signalling cascade
- The old view of asthma, 1960s to 1970s
- Asthma pathophysiology
- Asthma death
- IL-4 in mast cell asthmatic bronchial biopsy
- Mast cells, IL-4 cytokines distribution
- Mast cells, IL-5 and -6 cytokines distribution
- Human mast cells cytokines and chemokines
- Mast cell interactions in asthma pathogenesis
- Cytokines expression depends on stimulus
- Mast cells are continuously activated in asthma
- Mast cell mediator concentrations in BAL
- Spontaneous histamine release
- Tryptase and IL-4 mRNA in bronchial mucosa
- Mast cells morphological appearance in asthma
- Effect of salmeterol on inflammatory mediators
- Regular use of beta-2 agonists
- c-kit - stem cell factor axis in asthma
- Effect of SCF on beta-2 agonists in vitro
- SCF induces beta-2-adrenoceptor phosphorylation
- SCF induces beta-adrenoceptor internalisation
- Anti-SCF strategies
- Mast cell distribution in airways
- Mast cells express IL-6
- Mast cells and degranulating state correlation
- Asthma vs. Eosinophilic bronchitis (1)
- Asthma vs. Eosinophilic bronchitis (2)
- Mast cells tryptase expression in asthma
- Mast cell within bronchial smooth muscle
- Mast cells in the ASM bundle
- Mast cell infiltration of airway smooth muscle
- Mast cells localised to ASM in asthma
- Mast cell phenotype in ASM
- Mast cell morphology in airway smooth muscle
- Asthma vs. Eosinophilic bronchitis - summary
- Mast cell - ASM interactions
- Mast cell - ASM interactions, hypothesis
- Human lung mast cell isolation
- Mast cell migration
- CXCR3 is expressed by mast cells in ASM
- CXCL-10 (IP-10) is chemotactic for mast cells
- CXCL-10 (IP-10) release in asthma
- Supernatant lung mast cell migration induction
- CXCL10 immunostaining: normal/asthmatic ASM
- Migration summary
- Inhibiting human mast lung mast cell chemotaxis
- Mast cell activation: CRACM/Orai and KCa3.1
- Effect of KCa3.1 blockade on mast cell migration
- Specific chemokine targeting
- Human lung mast cells adhere to ASM
- Mast cell survival
- CADM1 and CD117 physical interaction
- HLMC-ASM co-culture effect
- Mast cells effect on alpha-smooth muscle actin
- HLMC-ASM co-culture summary
- Summary
- Potential inhibition targets
- Acknowledgements
Topics Covered
- Mast cell biology
- Mast cell cytokines
- Mast cell activation in asthma
- Mast cell tissue microlocalisation in asthma
- Mast cell-airway smooth muscle interactions as a key feature of asthma
Links
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Talk Citation
Bradding, P. (2011, December 29). The role of mast cells in asthma [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 13, 2024, from https://doi.org/10.69645/FARU6121.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Peter Bradding, Consultant: Icagen, Inc. Speaker’s Bureau: GlaxoSmithKline, AstraZeneca, Novartis Grant/Research Support (Principal Investigator): Boehringer-Ingelheim, Genentech
A selection of talks on Respiratory Diseases
Transcript
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0:00
I'm Peter Bradding, Professor of Respiratory Medicine,
in the Department of Infection,
Immunity and Inflammation at
the Institute for Lung Health at the University of Leicester, UK,
based at the Glenfield Hospital in Leicester.
In this lecture, I'm going to discuss the role
of mast cells in the pathophysiology of asthma.
0:22
In this lecture, I will start by giving a little background into
basic mast cell biology before looking at the role of
mast cells in the pathophysiology of asthma from a historical perspective,
and then I will move on to discuss more recent advances.
0:40
Mast cells originate in the bone marrow.
They are nonspecific mononuclear cells expressing CD34 and CD117 or c-kit,
stem cell factor receptor.
These mononuclear cells mobilize the peripheral blood through poorly defined mechanisms,
and then are recruited to the tissue under the influence of various chemoattractants.
There's only one stays mononuclear cells into the tissues that they then
differentiate and mature into the typical tissue mast cell.
This occurs particularly under the influence of
growth factors especially stem cell factor,
but also through cell-cell interactions and interactions with tissue matrix.
1:24
Mast cells have been implicated in many diverse biological processes.
In health, they have been implicated in growth and development,
wound healing, and host defense,
particularly to bacterial infection.
They've also been implicated in many diseases including allergy and asthma;
fibrotic diseases such as pulmonary fibrosis and systemic sclerosis;
rheumatoid disease, multiple sclerosis,
and atherosclerosis, which just highlights a few diverse examples.
This slide shows the typical morphology of