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1. Introduction
2. Molecular redundancy
3. Redundant molecules replace disrupted genes
4. Genomic structure of the CD44 molecule
5. CD44 - alternative splicing
6. CD44 ligands
7. Dr. Lora Eshkar Sebban
8. CD44 functions
9. The three steps model of Dr. Springer
10. Rolling and migration of CD44-positive cells
11. The migration process
12. Anti-CD44 mAbs reduce pathological activities
13. Anti-CD44 mAbs reduce diabetogenic activity
14. Anti-CD44 mAbs reduce arthritic activity
15. Generation of CIA model experimental protocol
16. In-vivo efficacy in CIA model
17. The CD44-dependency of CIA
18. CIA is a CD44-dependent disease
19. Effect of type 2 collagen injection
20. CD44 DNA analysis of CD44-deficient mice
21. Flow cytometry: CD44-deficient mice spleen cells
22. Sensitivity to CIA: CD44-knockout vs. WT mice
23. Histopathology of joints from CD44+/+ and -/- mice
24. Photo imaging of histochemical staining revealed
25. CD44-deficient mice: hyaluronic acid accumulation
26. Anti-CD44 mAb inhibits CIA in WT DBA/1 mice
27. Support of collagen-induced arthritis in mice
28. Inhibition of CIA by hyaluronidase in WT mice
29. Inhibition of CIA in WT and CD44-K/O mice
30. CD44-deficient spleen cells bind to HA
31. HA-binding protein compensates for CD44 in mice
32. The HA binding protein that compensates for CD44
33. RHAMM: receptor for hyaluronan-mediated motility
34. RHAMM reagents
35. RHAMM regulation in CD44-deficient mice (1)
36. RHAMM regulation in CD44-deficient mice (2)
37. Transwell migration assay
38. Spleen cells migration from CD44-deficient mice
39. Anti-RHAMM inhibits mice spleen cells migration
40. Anti-RHAMM mAb and soluble RHAMM inhibit CIA
41. CD44 affect the disease activity
42. CD44 deletion affect embryonic development
43. RHAMM regulation after CD44 genetic deletion
44. RHAMM functions
45. Interpretation of the redundancy mechanism
46. Disease-specific CD44 (1)
47. Disease-specific CD44 (2)
48. Exploring CD44 isoforms
49. Genomic sequence of: V4/V5 junction, CD44vRA
50. Single amino acid exchange and proteins function
51. Levels of CD44vRA Abs in mice splenocytes
52. Binding F8:33 anti-CD44 mAb to synovial cells
53. Synovial fluid cells from an RA and OA patient
54. F8:33 anti-CD44vRA mAb bindings
55. Does anti-CD44vRA Ab promote apoptosis?
56. Anti-CD44vRA mAb induces apoptosis in RA (1)
57. Anti-CD44vRA mAb induces apoptosis in RA (2)
58. Disease-specific CD44
59. Conclusions
60. Acknowledgements
61. Albert Einstein's wisdom...
62. References
63. References

Topics Covered

• CD44 and RHAMM (receptor for hyaluronan-mediated motility) are cell adhesion and cell homing receptors
• Cell migration
• Generation of different CD44 isoforms
• RHAMM also supports cell motility and is engaged in intracellular signalling
• Collagen-induced arthritis and type 1 diabetes are CD44-dependent pathologies
• Molecular redundancy
• CD44 targeting by anti-CD44 mAb may eradicate not only malignant cells and destructive inflammatory cells but also CD44-expressing normal cells which are committed to physiological functions

Links

Series:

• Autoimmunity

Categories:

• Diseases, Disorders & Treatments
• Genetics & Epigenetics
• Immunology

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Naor, D. (2007, October 1). CD44 genetic deletion enhances and CD44 antibody targeting attenuates autoimmune inflammation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved July 9, 2020, from https://hstalks.com/bs/213/.

Publication History

• Published on October 1, 2007
• Reviewed on November 28, 2017

Financial Disclosures

• Prof. David Naor has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.