Please wait while the transcript is being prepared...
0:00
This talk introduces Eliza,
enzyme linked
immunosorbent assay,
using it as a basis for
further exploration
of the principles
of the Eliza technique,
including its stepwise
procedure and reliance on
antibody specificity and
enzyme based detection.
We will examine the
different Eliza formats,
direct, indirect,
and sandwich and
discuss how each
is optimized for specific
analytical needs.
Key applications in
clinical diagnostics
and biomedical research
will be highlighted,
along with recent
advancements that
enhance Eliza's versatility
and sensitivity.
Elsa, the enzyme linked
immunosorbent assay
is an indispensable technique in
immunology, diagnostics,
and research.
ElsA is a plate based assay
designed to detect and
quantify soluble substances,
including proteins, antibodies,
hormones, and cytokines.
Thanks to its sensitivity,
specificity, and adaptability,
Elsa is used to
diagnose diseases,
monitor immune responses, and
measure biomolecules
in complex mixtures.
The success of analyser
depends on a sequence of
coordinated steps
supporting its specificity
and sensitivity.
It begins with coating,
where an antigen or
antibody is immobilized on
a polystyrene microplate.
Blocking follows using
unrelated proteins to
fill uncoated sites and
prevent non specific
interactions.
Binding occurs as
the sample is added,
allowing target analytes
to bind specifically.
Detection uses an enzyme
conjugated antibody or antigen,
