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Navigable Slide Index

1. Introduction
2. The first cell
3. Investigating function
4. Gating versus permeation
5. Tools for investigating function: electrophysiology
6. Tools for investigating function: blockers
7. Blocker
8. State dependent access
9. Foot in the door
10. An example - fast block
11. Tools for investigating function: mutagenesis
12. Point mutations
13. Channel topology
14. The pore region in sodium channels
15. TTX attraction to neutral aromatic residue
16. QM/MM model
17. Chimeras
18. Examples of chimeras: KcsA and Shaker
19. Examples of chimeras: sodium and potassium (1)
20. Examples of chimeras: sodium and potassium (2)
21. Tools for investigating function: SCAM
22. Substituted cysteine accessibility method
23. Methanethiosulfonate reagents
24. Substituted cysteine accessibility method
25. Designing a voltage-gated channel
26. S4 segment movement
27. Tools for investigating: fluorescence probes
28. FluoProbes
29. Tools for investigating function: simulations
30. Conclusions

Topics Covered

• Methods used to study the biophysical properties of ion-channel function
• Relationship between ion channel structure and function

Links

Series:

• Ion Channels

Categories:

• Biochemistry

Talk Citation

Publication History

• Published on February 28, 2011

Financial Disclosures

• Dr. Richard Horn has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.