• The Discovery of Protein Phosphorylation
• 45 min
  1. Phosphorylase and the origin of reversible protein phosphorylation

  • Prof. Edmond Fischer
• 21 min
  2. Three decades of protein phosphorylation and cancer: the identification and characterization of the src gene product

  • Prof. Raymond Erikson
• Protein Kinase Cascades
• 32 min
  3. Role of the PI 3-kinase signaling pathway in cell regulation and human disease

  • Prof. Lew Cantley
• The Modulation of Protein Function by Phosphorylation
• 33 min
  4. Two is the key to 14-3-3: dimeric mechanical signaling devices

  • Prof. Carol MacKintosh
• Protein Phosphatases
• 38 min
  5. Structure and mechanisms of protein phosphatases

  • Prof. David Barford
• 45 min
  6. Protein tyrosine phosphatases

  • Prof. Jack Dixon
• 49 min
  7. The regulation of MAP kinase signalling by dual-specificity protein phosphatases

  • Prof. Steve M. Keyse
• 22 min
  8. Protein phosphatase inhibitors: a distillation of cell signaling research and water toxicology

  • Prof. Carol MacKintosh
• The Structures of Protein Kinases
• 45 min
  9. Protein kinase structure, function and regulation

  • Prof. Susan Taylor
• 42 min
  10. The structural basis for the modulation of protein function by protein phosphorylation

  • Prof. Dame Louise N. Johnson
• Biological Systems that are Regulated by Reversible Phosphorylation
• 50 min
  11. Protein phosphorylation and the control of protein synthesis

  • Prof. Christopher Proud
• 62 min
  12. The role of phosphorylation in mediating cellular responses to DNA damage: the ATM-mediated DNA damage response

  • Prof. Yosef Shiloh
• 40 min
  13. Roles of AMPK in energy homeostasis and nutrient sensing

  • Prof. Grahame Hardie
• 56 min
  14. Serine kinases and T lymphocyte biology

  • Prof. Doreen Cantrell
• 30 min
  15. The interplay between protein phosphorylation and ubiquitylation in the NF-κB pathway

  • Prof. Zhijian 'James' Chen
• 58 min
  16. SMAD phosphorylation and the TGF-beta pathway

  • Prof. Joan Massagué
• Protein Kinases and Human Disease
• 58 min
  17. Function and regulation of the PDK1 kinase

  • Prof. Dario Alessi
• 63 min
  18. LKB1 pathway and its role in cancer

  • Prof. Dario Alessi
• 51 min
  19. WNK1 pathway and its role in regulating hypertension

  • Prof. Dario Alessi
• 33 min
  20. The hyperphosphorylation of tau and Alzheimer's disease

  • Prof. Michel Goedert
• Protein Kinases as Targets for the Development of Anti-Cancer Drugs
• 68 min
  21. PI3K/AKT signaling in cancer

  • Prof. Neal Rosen
• 37 min
  22. RAS and RAF signaling in melanoma: biology and therapies

  • Prof. Richard Marais
• 38 min
  23. The mTOR kinase as a target for anti-cancer drugs

  • Prof. David Sabatini
• Archived Lectures *These may not cover the latest advances in the field
• 51 min
  24. Modular protein-protein interactions provide a general mechanism to organize dynamic cellular systems

  • Prof. Tony Pawson
• 38 min
  25. AMP-activated protein kinase: regulating cellular and whole body energy balance

  • Prof. Grahame Hardie

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. The 14-3-3 dimer
3. Plants photosynthesise
4. 14-3-3 inhibits nitrate reductase
5. Mutated nitrate reductase and NO levels
6. Other functions of 14-3-3
7. Plant and human 14-3-3s are identical
8. Human and plant 14-3-3-binding proteins
9. 14-3-3 dimers as mechanical devices
10. 14-3-3 alter tyrosine hydroxylase conformation
11. A 14-3-3 clamp masks DNA binding in FOXO4
12. A 14-3-3 may clip flanking regions
13. 14-3-3 may act as an adapter of two proteins
14. Phosphorylated 14-3-3-binding sites
15. Many kinases phosphorylate 14-3-3 binding sites
16. IGF1/PI3 and 14-3-3-binding site phosphorilation
17. 14-3-3 capture and release with isotope labelling
18. Overlapping substrates of PKB/Akt and p90RSK
19. Patterns in 14-3-3-binding phosphoproteome (1)
20. Patterns in 14-3-3-binding phosphoproteome (2)
21. Function of 14-3-3 in glucose uptake in muscles
22. 14-3-3 binding sites in AS160 and TBC1D1
23. Yin-Yang regulation of TBC1D1 and AS160
24. Regulating glucose trafficking in different tissues
25. 14-3-3 as digital logic gate
26. 14-3-3 dimer as logic gate or coincidence detector
27. Hypothesis: 14-3-3 dimer as evolutionary device
28. Summary
29. Thanks to....

Topics Covered

• 14-3-3s dock onto specific sites phosphorylated by AGC and CAMK kinases
• 14-3-3s have hundreds of phosphoprotein partners
• 14-3-3 dimers as mechanical levers and clamps, and 'coincidence detectors'
• Cellular regulation of the 14-3-3-binding phosphoproteome
• Case studies including plant leaf responses to darkness and human cell responses to insulin

Links

Series:

• Protein Phosphorylation

Categories:

• Biochemistry

Talk Citation

Publication History

• Published on November 30, 2010

Financial Disclosures

• Prof. Carol MacKintosh has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.