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1. Introduction
2. The 14-3-3 dimer
3. Plants photosynthesise
4. 14-3-3 inhibits nitrate reductase
5. Mutated nitrate reductase and NO levels
6. Other functions of 14-3-3
7. Plant and human 14-3-3s are identical
8. Human and plant 14-3-3-binding proteins
9. 14-3-3 dimers as mechanical devices
10. 14-3-3 alter tyrosine hydroxylase conformation
11. A 14-3-3 clamp masks DNA binding in FOXO4
12. A 14-3-3 may clip flanking regions
13. 14-3-3 may act as an adapter of two proteins
14. Phosphorylated 14-3-3-binding sites
15. Many kinases phosphorylate 14-3-3 binding sites
16. IGF1/PI3 and 14-3-3-binding site phosphorilation
17. 14-3-3 capture and release with isotope labelling
18. Overlapping substrates of PKB/Akt and p90RSK
19. Patterns in 14-3-3-binding phosphoproteome (1)
20. Patterns in 14-3-3-binding phosphoproteome (2)
21. Function of 14-3-3 in glucose uptake in muscles
22. 14-3-3 binding sites in AS160 and TBC1D1
23. Yin-Yang regulation of TBC1D1 and AS160
24. Regulating glucose trafficking in different tissues
25. 14-3-3 as digital logic gate
26. 14-3-3 dimer as logic gate or coincidence detector
27. Hypothesis: 14-3-3 dimer as evolutionary device
28. Summary
29. Thanks to....

Topics Covered

• 14-3-3s dock onto specific sites phosphorylated by AGC and CAMK kinases
• 14-3-3s have hundreds of phosphoprotein partners
• 14-3-3 dimers as mechanical levers and clamps, and 'coincidence detectors'
• Cellular regulation of the 14-3-3-binding phosphoproteome
• Case studies including plant leaf responses to darkness and human cell responses to insulin

Links

Series:

• Protein Phosphorylation

Categories:

• Biochemistry

Talk Citation

MacKintosh, C. (2010, November 30). Two is the key to 14-3-3: dimeric mechanical signaling devices [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/QXLU4092.
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Publication History

• Published on November 30, 2010

Financial Disclosures

• Prof. Carol MacKintosh has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.