• The Discovery of Protein Phosphorylation
• 45 min
  1. Phosphorylase and the origin of reversible protein phosphorylation

  • Prof. Edmond Fischer
• 21 min
  2. Three decades of protein phosphorylation and cancer: the identification and characterization of the src gene product

  • Prof. Raymond Erikson
• Protein Kinase Cascades
• 32 min
  3. Role of the PI 3-kinase signaling pathway in cell regulation and human disease

  • Prof. Lew Cantley
• The Modulation of Protein Function by Phosphorylation
• 33 min
  4. Two is the key to 14-3-3: dimeric mechanical signaling devices

  • Prof. Carol MacKintosh
• Protein Phosphatases
• 38 min
  5. Structure and mechanisms of protein phosphatases

  • Prof. David Barford
• 45 min
  6. Protein tyrosine phosphatases

  • Prof. Jack Dixon
• 49 min
  7. The regulation of MAP kinase signalling by dual-specificity protein phosphatases

  • Prof. Steve M. Keyse
• 22 min
  8. Protein phosphatase inhibitors: a distillation of cell signaling research and water toxicology

  • Prof. Carol MacKintosh
• The Structures of Protein Kinases
• 45 min
  9. Protein kinase structure, function and regulation

  • Prof. Susan Taylor
• 42 min
  10. The structural basis for the modulation of protein function by protein phosphorylation

  • Prof. Dame Louise N. Johnson
• Biological Systems that are Regulated by Reversible Phosphorylation
• 50 min
  11. Protein phosphorylation and the control of protein synthesis

  • Prof. Christopher Proud
• 62 min
  12. The role of phosphorylation in mediating cellular responses to DNA damage: the ATM-mediated DNA damage response

  • Prof. Yosef Shiloh
• 40 min
  13. Roles of AMPK in energy homeostasis and nutrient sensing

  • Prof. Grahame Hardie
• 56 min
  14. Serine kinases and T lymphocyte biology

  • Prof. Doreen Cantrell
• 30 min
  15. The interplay between protein phosphorylation and ubiquitylation in the NF-κB pathway

  • Prof. Zhijian 'James' Chen
• 58 min
  16. SMAD phosphorylation and the TGF-beta pathway

  • Prof. Joan Massagué
• Protein Kinases and Human Disease
• 58 min
  17. Function and regulation of the PDK1 kinase

  • Prof. Dario Alessi
• 63 min
  18. LKB1 pathway and its role in cancer

  • Prof. Dario Alessi
• 51 min
  19. WNK1 pathway and its role in regulating hypertension

  • Prof. Dario Alessi
• 33 min
  20. The hyperphosphorylation of tau and Alzheimer's disease

  • Prof. Michel Goedert
• Protein Kinases as Targets for the Development of Anti-Cancer Drugs
• 68 min
  21. PI3K/AKT signaling in cancer

  • Prof. Neal Rosen
• 37 min
  22. RAS and RAF signaling in melanoma: biology and therapies

  • Prof. Richard Marais
• 38 min
  23. The mTOR kinase as a target for anti-cancer drugs

  • Prof. David Sabatini
• Archived Lectures *These may not cover the latest advances in the field
• 51 min
  24. Modular protein-protein interactions provide a general mechanism to organize dynamic cellular systems

  • Prof. Tony Pawson
• 38 min
  25. AMP-activated protein kinase: regulating cellular and whole body energy balance

  • Prof. Grahame Hardie

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. 55 years ago
3. Studying glycogen phosphorylase
4. Degradation of glycogen - adrenaline
5. Fight or flight
6. Insulin, glycogen synthase and glucagon
7. Louis Leloir
8. How this research started
9. Regulation of phosphorylase
10. Photograph of the Coris
11. Phosphorylase showed no requirement for AMP
12. Edwin Krebs - hesitation
13. Cori's preparation of muscle phosphorylase (1)
14. Purification of phosphorylase
15. Edwin Krebs - Cori's preparation
16. Ca2+ contamination is responsible for activation
17. Edwin Krebs - ashes experiment
18. What was phosphorylated?
19. Activation-inactivation cycle of phosphorylase
20. Edwin Krebs - JBC reviewers
21. Knowledge of phospho proteins in 1950
22. Ca2+ involvement in the crude extracts
23. Phosphorylase kinase is also phosphorylated
24. Sutherland
25. cAMP and hormonal control of glycogenolysis
26. Ca2+ and muscle contraction
27. Single fiber measurement method
28. Tension and phosphorylase kinase activity
29. Effect of the sarcoplasmic reticulum
30. The complete cycle of Ca2+
31. Ubiquitous protein phosphorylation
32. Phosphorylase was a good protein to work on
33. Reciprocal control
34. Control of glycogen synthesis and breakdown
35. Mechanism of activation of GSK-3
36. Not everybody believed in regulation by phos.
37. Looking back at the early days
38. The world - before and after
39. Thank you

Topics Covered

• A historical account of the discovery of reversible protein phosphorylation
• Video clips of the late Edwin Krebs speaking about our early work
• Reversible protein phosphorylation represents one of the most prevalent mechanism by which cellular events are regulated
• Establishment of the first hormonal cascade of successive enzymatic reactions initiated by cAMP

Links

Series:

• Protein Phosphorylation

Categories:

• Biochemistry

Talk Citation

Publication History

• Published on November 30, 2010

Financial Disclosures

• Prof. Edmond Fischer has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.