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Navigable Slide Index

1. Introduction
2. 55 years ago
3. Studying glycogen phosphorylase
4. Degradation of glycogen - adrenaline
5. Fight or flight
6. Insulin, glycogen synthase and glucagon
7. Louis Leloir
8. How this research started
9. Regulation of phosphorylase
10. Photograph of the Coris
11. Phosphorylase showed no requirement for AMP
12. Edwin Krebs - hesitation
13. Cori's preparation of muscle phosphorylase (1)
14. Purification of phosphorylase
15. Edwin Krebs - Cori's preparation
16. Ca2+ contamination is responsible for activation
17. Edwin Krebs - ashes experiment
18. What was phosphorylated?
19. Activation-inactivation cycle of phosphorylase
20. Edwin Krebs - JBC reviewers
21. Knowledge of phospho proteins in 1950
22. Ca2+ involvement in the crude extracts
23. Phosphorylase kinase is also phosphorylated
24. Sutherland
25. cAMP and hormonal control of glycogenolysis
26. Ca2+ and muscle contraction
27. Single fiber measurement method
28. Tension and phosphorylase kinase activity
29. Effect of the sarcoplasmic reticulum
30. The complete cycle of Ca2+
31. Ubiquitous protein phosphorylation
32. Phosphorylase was a good protein to work on
33. Reciprocal control
34. Control of glycogen synthesis and breakdown
35. Mechanism of activation of GSK-3
36. Not everybody believed in regulation by phos.
37. Looking back at the early days
38. The world - before and after
39. Thank you

Topics Covered

• A historical account of the discovery of reversible protein phosphorylation
• Video clips of the late Edwin Krebs speaking about our early work
• Reversible protein phosphorylation represents one of the most prevalent mechanism by which cellular events are regulated
• Establishment of the first hormonal cascade of successive enzymatic reactions initiated by cAMP

Links

Series:

• Protein Phosphorylation

Categories:

• Biochemistry

Talk Citation

Fischer, E. (2010, November 30). Phosphorylase and the origin of reversible protein phosphorylation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 19, 2025, from https://doi.org/10.69645/ROXK9891.
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Publication History

• Published on November 30, 2010

Financial Disclosures

• Prof. Edmond Fischer has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.