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Printable Handouts
Navigable Slide Index
- Introduction
- Historical malaria in Europe
- Anopheles mosquitoes and malaria
- Anophelism without malaria
- Clues to the puzzle
- Distribution of Anopheles atroparvus
- More clues to the puzzle
- Identification of blood meal sources
- The Anopheles maculipennis complex
- Key biological characteristics
- What is a species complex?
- Examples of species complexes
- Other complexes
- How are they identified?
- Anopheles gambiae complex
- Identification of gambiae complex
- Genetic crosses
- Cytogenetics
- Insecticide resistance in the gambiae complex
- Techniques for investigating species complexes
- Proteins as markers
- Example of allozyme analysis
- Disadvantages of proteins as markers
- DNA-based methodologies
- How PCR works
- Step 1: denaturation
- Step 2: annealing
- Step 3: extension
- Repeated cycles amplify fragment of interest
- Amplification of genomic DNA fragment
- PCR reaction components
- Advantages to PCR-based diagnostic methods
- Distinguishing gambiae species by diagnostic PCR
- Target sequences
- Cytochrome oxidase I gene
- Ribosomal DNA
- Primer design for diagnostic PCR
- Example of diagnostic PCR
- Validation
- Multiplex PCR
- Identification of new cryptic species
- Evolution of species complexes
- Anopheles sundaicus complex
- Human expansion
- Humans and agriculture
- Molecular forms
- Ecological speciation
- Ecotypic speciation
- Bamako
- Species complexes and gene flow
- Thank you
Topics Covered
- History and the anopheline mosquito species complexes
- Methods of identification of species complexes (behavioral, genetic, chromosomal)
- Molecular tools for diagnosis of species
- Evolution of species complexes
- Ecological and ecotypic speciation
Talk Citation
Paskewitz, S. (2010, October 26). Species complexes: confusion in identifying the true vectors of malaria and other parasites [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 23, 2024, from https://doi.org/10.69645/RFDH7399.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Susan Paskewitz has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Species complexes: confusion in identifying the true vectors of malaria and other parasites
A selection of talks on Infectious Diseases
Transcript
Please wait while the transcript is being prepared...
0:00
This presentation
will cover the topic of Species
Complexes- Confusion
in Identifying the True
Vectors of Malaria
and Other Parasites.
My name is Susan Paskewitz.
I'm a professor and medical
entomologist in the Department
of Entomology at the
University of Wisconsin.
0:19
Let's begin with a
historical example
that illustrates the problem.
Malaria was an old
scourge in Europe,
with patterns of transmission that
varied from constant incidence
in hyperendemic areas to periodic
outbreaks in epidemic zones.
0:37
Following the momentous
discovery in 1898
that Anopheles mosquitoes
transmit malaria,
Anopheles maculipennis was
circumstantially incriminated
as the most likely vector in Europe.
This mosquito was originally
described and named from specimens
that had been collected
in Germany around 1818.
And for the next 100 years,
it was believed that Anopheles
maculipennis was the major
Anophele mosquito in Europe,
with a wide distribution
through most countries.
1:10
But one of the most serious
criticisms of the theory
that Anopheles transmitted
malaria was that high densities
of these mosquitoes
were frequently reported
in areas with no
malaria transmission.
This pattern was called
anophelism without malaria.
Equally curious was the
disappearance of malaria
from certain endemic areas of Europe
without any effort to eradicate it,
and despite high
densities of Anopheles.
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