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Printable Handouts
Navigable Slide Index
- Introduction
- Outline of the talk
- Stability and inheritance of DNA methylation
- Genome-wide analysis of DNA methylation
- Genome-wide DNA methylation profiling
- MBD proteins bind to CPG methylated DNA
- The MBD2/3 family
- MBD2b binding to methylated DNA by MBD3L1
- Methylated-CpG Island Recovery Assay (MIRA)
- Specificity of MIRA
- Efficiency of the MIRA pull-down
- Verification by bisulfite conversion
- MIRA-chip and MIRA-seq.
- MIRA versus MeDIP: difference in sensitivity
- DNA methylation in normal B cells
- DNA methylation in normal B cells: CD19 locus
- Methylation at the HOXA and XIST loci
- Signal and methylation density
- Methylation along chromosomes (1)
- Methylation along chromosomes (2)
- More than 42,000 methylation peaks
- Methylation peaks and methylated CpG islands
- Gene expression and promoter methylation (1)
- Gene expression and promoter methylation (2)
- Methylation controls alternative promoters
- Gene expression and intragenic methylation
- DNA methylation peak at the 5' and 3' ends
- DNA methylation changes in cancer
- Lung tumors analyzed on CpG island arrays
- DNA methylation in lung tumors
- Homeobox genes as methylation targets
- Homeobox genes are methylation hotspots
- CpG islands methylation near homeobox genes
- Methylation of Polycomb target genes
- Polycomb and DNA methylation in cancer
- Inflammation, DNA methylation and cancer (1)
- Inflammation, DNA methylation and cancer (2)
- Mouse IBD model
- CpG island methylation in GPX-1/2 KO mice
- Methylation in the ileum of GPX1/2-KO mice (1)
- Polycomb genes methylation in inflammation (1)
- Polycomb genes methylation in inflammation (2)
- DNA methylation and gene expression levels
- Genetic backgrounds/aging and inflammation
- Methylation in the ileum of GPX1/2-KO mice (2)
- Gene methylation in cancer and inflamed tissue
- Aging and inflammation in DNA methylation
- Conclusions
- Acknowledgements
Topics Covered
- DNA methylation in cancer in general
- Lung cancer
- Intestinal cancer
- Inflammation
- Method for genome-wide DNA methylation profiling
- Association of the Polycomb complex with DNA methylation in inflammation and cancer
Links
Series:
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Therapeutic Areas:
Talk Citation
Pfeifer, G. (2020, May 1). DNA methylation, inflammation and cancer [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 6, 2024, from https://doi.org/10.69645/KCKH5863.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Gerd Pfeifer has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
DNA methylation, inflammation and cancer
A selection of talks on Oncology
Transcript
Please wait while the transcript is being prepared...
0:00
My name is Gerd Pfeiffer.
The topic of my talk is DNA methylation,
inflammation, and cancer.
0:08
The talk will be divided into four parts,
in part one, a method for
DNA methylation analysis,
genome wide will be described.
In the second part,
data on the DNA methylation patterns
in B cells will be reported.
In part three, I will discuss our data
on the inner methylation in lung cancer.
And in the last part,
the relationship between inflammation,
DNA methylation and cancer and targeting
of specific genes will be discussed.
0:38
DNA methylation patterns in mammalian
cells are generally quite stable.
During DNA replication,
methylation at CpG sites is copied
by DNA methyltransferase one.
Methylation can also be introduced de
novo or methylation can be lost by DNA
demethylation processes, either active or
passive DNA demethylation.
These mechanisms can be
altered in diseased tissue.
1:02
There's many different methods to analyze
DNA methylation on a genome-wide scale.
Some are based on methylation
sensitive restriction enzymes.
They're shown here in panels A,
B and C, the information can be
detected by two dimensional gel
electrophoresis by PCR or by micro arrays.
Another method used is an antibody against
methylated cytosine is shown here in
panel D and it's called Methyl-DIP.
This method is like immunoprecipitation
and the methylated immunoprecipitated DNA
can be analyzed either by high throughput
sequencing or by microarray chip analysis.
Another way to look at methylated genes
is to use a methylation inhibitor,
5-Aza-cytidine or DAC.
Genes that become reactivated by DAC
treatment shown here in panel E and
then be scored and methylation must
then be confirmed by different,
more specific methods.
We developed a method for
genome wide DNA methylation profiling,