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Navigable Slide Index

1. Introduction
2. Cystic fibrosis
3. Cystic fibrosis: survival
4. Positional cloning
5. Linkage analysis
6. Pedigree analysis
7. Further linkage analysis
8. Step 1: chromosome walking to make a contig
9. Chromosome jumping
10. The resulting contig
11. Step 2: Identification of candidate genes: Zoo blot
12. Zoo blots
13. Identification of candidate genes: Northern blots
14. Northern blots
15. Identifying the cystic fibrosis gene: conclusions
16. Mutations in CFTR
17. Identifying the cystic fibrosis gene: summary
18. Remaining questions
19. Duchenne muscular dystrophy
20. Translocation between chromosomes X and 21
21. Subtractive cloning
22. Subtractive cloning using PERT
23. Role of dystrophin
24. References

Topics Covered

• Cystic fibrosis
• Survival
• Positional cloning
• Linkage analysis
• Pedigree analysis
• Zoo blots
• Northern blots
• Mutations
• Duchenne muscular dystrophy
• Translocation
• Subtractive cloning between chromosome X and chromosome 21
• Role of Dystrophin

Links

Series:

• Introduction to Human Genetics

Categories:

• Clinical Practice
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on December 28, 2009
• Archived on September 8, 2024

Financial Disclosures

• Prof. Jonathan Wolfe has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.