• Models of Investigation
• 41 min
  1. Antifungal innate immunity in C. elegans

  • Dr. Jonathan Ewbank
• 44 min
  2. The anti-microbial defense of Drosophila: a paradigm for innate immunity

  • Prof. Jules Hoffmann
• 38 min
  3. Phagocytosis in the fruit fly, Drosophila melanogaster

  • Dr. Lynda Stuart
• 62 min
  4. Innate immune sensing and response

  • Prof. Bruce Beutler
• 61 min
  5. Macrophages and systems biology

  • Prof. David Hume
• Cell Types and Recruitment
• 71 min
  6. Leukocyte recruitment in vivo

  • Prof. Paul Kubes
• 51 min
  7. Chemokines and inflammation: a critical assessment of therapeutic targets

  • Dr. David Greaves
• 42 min
  8. Eosinophils

  • Prof. Tim Williams
• 63 min
  9. Dendritic cells: linking innate to different forms of adaptive immunity

  • Prof. Ralph Steinman
• 25 min
  10. Plasmacytoid dendritic cells: sensing nucleic acids in viral infection and autoimmunity

  • Prof. Yong-Jun Liu
• 61 min
  11. Innate-like lymphocytes 1

  • Prof. Adrian Hayday
• 41 min
  12. Innate-like lymphocytes 2

  • Prof. Adrian Hayday
• Recognition and Signaling
• 50 min
  13. Colony stimulating factor-1 regulation of macrophages in development and disease

  • Prof. E. Richard Stanley
• 42 min
  14. Fc receptors: linking innate and acquired immunity

  • Prof. Ken G C Smith
• 39 min
  15. Phagocytosis

  • Prof. Joel Swanson
• 44 min
  16. Clearance of apoptotic cells and the control of inflammation

  • Prof. Sir John Savill
• 38 min
  17. Signaling by innate immune receptors

  • Prof. Michael Karin
• 41 min
  18. Nuclear receptors at the crossroads of inflammation and atherosclerosis

  • Prof. Christopher Glass
• Modulation of Effector Responses
• 22 min
  19. Humoral innate immunity and the acute phase response 1

  • Prof. Alberto Mantovani
• 42 min
  20. Humoral innate immunity and the acute phase response 2

  • Prof. Alberto Mantovani
• 54 min
  21. Cytokines regulating the innate response

  • Prof. Anne O’Garra
• 39 min
  22. Arginase and nitric oxide

  • Dr. Peter Murray
• 38 min
  23. Novel lipid mediators in resolution of inflammation

  • Prof. Charles Serhan
• 69 min
  24. Resolvins activate inflammation-resolution programs: a systems approach to resolution

  • Prof. Charles Serhan
• 42 min
  25. Cationic peptides in innate immunity

  • Dr. Dawn Bowdish
• 27 min
  26. Iron metabolism and innate immunity

  • Prof. Tomas Ganz
• Pathogen-Host Interactions
• 46 min
  27. Innate recognition of viruses

  • Prof. Caetano Reis e Sousa
• 50 min
  28. Type I interferons in innate immunity to viral infections

  • Prof. Christine Biron
• 56 min
  29. HIV-1 and immunopathogenesis: innate immunity

  • Prof. Luis Montaner
• 53 min
  30. Understanding and combating tuberculosis

  • Prof. David Russell
• 34 min
  31. The taste of a fungus: recognition of Candida by the innate immune system

  • Prof. Neil Gow
• 51 min
  32. Innate immunity and malaria

  • Prof. Douglas Golenbock
• Health and Disease
• 39 min
  33. Innate immunity in children

  • Prof. David Speert
• 59 min
  34. From bench to bedside: evolution of anti-TNFalpha therapy in rheumatoid arthritis

  • Prof. Sir Ravinder Maini
• 33 min
  35. NOD-like receptors in innate immunity and inflammatory disease

  • Prof. Gabriel Nunez
• 38 min
  36. Paneth cells in innate immunity and inflammatory bowel disease

  • Prof. Satish Keshav
• 36 min
  37. Innate immunity in the brain in health and disease

  • Prof. V. Hugh Perry
• 54 min
  38. The fate of monocytes in atherosclerosis

  • Prof. Gwendolyn Randolph
• 49 min
  39. Macrophages, a cellular toolbox used by tumors to promote progression and metastasis

  • Prof. Jeffrey Pollard
• Archived Lectures *These may not cover the latest advances in the field
• 73 min
  40. Monocyte/macrophages in innate immunity

  • Prof. Emeritus Siamon Gordon
• 32 min
  41. Innate immunity in C. elegans

  • Dr. Jonathan Ewbank
• 51 min
  42. Antigen processing and presentation: modulation by innate immune signals

  • Prof. Colin Watts
• 39 min
  43. NLR genes: infection, inflammation and vaccines

  • Prof. Jenny Ting
• 63 min
  44. Manipulation of innate immune response: lessons from shigella

  • Prof. Philippe Sansonetti
• 35 min
  45. Innate immunity of the lung and adaptation to air breathing at birth

  • Prof. Jeffrey Whitsett

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Malaria: the facts in 2007
3. The pathology of malaria is driven by cytokines
4. Life cycle of the malarial parasite
5. Why do humans get fever during malaria?
6. The search for the "malaria toxin" (1)
7. The search for the "malaria toxin" (2)
8. Is it a component of the outer membrane?
9. Gram negative cell envelope - LPS
10. Is the malaria toxin just like LPS?
11. Glycosylphosphatidyl inositol anchor (GPI)
12. GPI anchors activate TLR2
13. Purified P.falciparum failed to induce cytokines
14. The major immune-stimulus in malaria is not GPI
15. What is hemozoin?
16. Pure hemozoin failed to induce cytokines
17. Hemozoin levels and disease severity
18. Hemozoin production
19. Purification of hemozoin with a magnetic column
20. Purified hemozoin
21. Hypothesis (1)
22. What is a Toll receptor?
23. Identification of Toll receptors (1)
24. "Toll"
25. Toll receptor and TIR domain
26. Identification of toll receptors (2)
27. Toll receptor role in innate immune response (1)
28. Toll receptor role in innate immune response (2)
29. Toll innate immune response in humans and flies
30. TLRs in humans, mice and flies
31. The purple sea urchin has 222 TLRs
32. Innate immune recognition system 1997
33. Innate immune recognition system 2007
34. Domains of TLR
35. Shizuo Akira (1)
36. Bacterial DNA is rich in non-methylated CpG
37. Shizuo Akira (2)
38. Hz activates IL1 production via TLR9/MyD88
39. Activity of hemozoin is destroyed by DNase
40. Thus, DNA is the cytokine inducing component
41. Is the DNA on hemozoin human or malarial?
42. PCR analysis shows that most DNA is malarial
43. Can malaria DNA actually stimulate cells?
44. The cell biology of TLR9 is complex
45. TLR9 translocates to the endosome
46. Does malaria DNA activate innate immunity?
47. Malaria DNA is stimulatory only with DOTAP (1)
48. Malaria DNA is stimulatory only with DOTAP (2)
49. The malaria genome contains 269 CpG repeats
50. Current hypothesis
51. But, most malaria DNA is AT-rich
52. Patients with malaria show IFN signature
53. Hemozoin/DNA activates IFN-beta production
54. Malaria genome contains the motif ATTTTTAC
55. AT-rich ODN mimic malarial DNA
56. AT-rich ODN activate IFN-beta production
57. AT-rich ODN activate IFN independently of TLRs
58. AT-rich ODN activation of IFN requires IRF1
59. Is the inflammasome involved in the recognition?
60. The inflammasome
61. AT-rich ODN activate IL-1beta production
62. Activation of IL-1beta is via caspase-1
63. Malarial DNA activates the inflammasome
64. Is the inflammasome involved in IFN Generation?
65. Activation of IFN requires Nalp3 but not ASC
66. Activation of IL-1beta requires caspase 1
67. Hemozoin DNA - from phagosome to cytosol
68. Leakage after phagocytosis of inert particles
69. Alzheimer's disease
70. Beta amyloid
71. A-beta induces IL-1beta production in microglia
72. Beta-amyloid causes lysosomal swelling (1)
73. Beta-amyloid causes lysosomal swelling (2)
74. Beta-amyloid causes lysosomal leakage
75. Conclusion
76. Hypothesis (2)
77. Summary
78. Thanks

Topics Covered

• Malaria causes systemic inflammation, morbidity and death
• Toxic mediators: GPI anchor, Hemozoin/DNA
• DNA motifs: CpG (TLR9) and AT-rich (unknown receptor)
• Mechanisms of malarial sepsis-like syndrome, cerebral malaria

Links

Series:

• Innate Immunity

Categories:

• Clinical Practice
• Immunology
• Microbiology

Therapeutic Areas:

• Immunology & Inflammation
• Infectious Diseases

Talk Citation

Publication History

• Published on June 30, 2009

Financial Disclosures

• Prof. Douglas Golenbock has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.