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1. Introduction
2. Talk topics
3. The kinetochore
4. Molecular composition of the human kinetochore
5. Kinetochore proteins assemble in a temporal order
6. CENP-F localization at different stages of G2
7. Defining temporal orders of assembly
8. FRAP studies and kinetochore
9. Turnover dynamics of kinetochore proteins
10. What is the kinetochore?
11. CENP-E: a kinesin like motor
12. CENP-E exhibits a dynamic localization pattern
13. CENP-E is essential for microtubule attachment
14. The HEC1/Ndc80 complex
15. Different phenotypes for various depletions
16. Three steps of the mitotic checkpoint pathway
17. Checkpoint proteins localize to kinetochores
18. The kinetochore and its relation to cancer
19. The mitotic checkpoint and cancer
20. Mitotic checkpoint genes are essential
21. Mitotic checkpoints and genome instability
22. The kinetochore as a mitosis specific target
23. Inhibitors of mitosis
24. Ansamycins are inhibitors of Hsp90
25. 17-AAG blocks assembly of CENP-H and I
26. Histone deacetylase inhibitors
27. HDI delays human tumor cell lines in mitosis
28. HDI interferes protein assembly onto kinetochore
29. Not all kinetochore localized proteins are affected
30. The depletion is not a result of degradation
31. FTI's block farnesylation of proteins
32. FTI-2153 alters the localization of CENP-F
33. Effect of FTI on lung cancer cells
34. Inhibitors of kinetochore/spindle proteins
35. Kinase inhibitors-Aurora and Plk1
36. Polo kinases - Plk1-4
37. Pros and cons of targeting the mitotic checkpoint
38. Linking chromosome missegregation to cell death
39. The checkpoint and apoptosis pathways
40. Hela cells depleted of hNuf2 accumulate in mitosis
41. Timelapse videos of mitotically arrested cells
42. Hela cells blocked in mitosis undergo apoptosis
43. Patient response
44. Conclusions and future directions

Topics Covered

• Mitosis is critical for cells to proliferate and is a major target of current anti-cancer drugs such as paclitaxel and vinca alkaloids
• Characterization of proteins essential for mitosis has made available the opportunity to develop new anti-mitotic agents
• Expanding the number of anti-cancer drug targets should significantly improve the response of tumors to chemotherapies

Links

Series:

• The Cell Division Cycle

Categories:

• Cancer
• Cell Biology
• Pharmaceutical Sciences

Therapeutic Areas:

• Oncology

Talk Citation

Yen, T. (2009, May 3). The kinetochore as a target for the development of mitosis specific anti-cancer drugs [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 19, 2024, from https://hstalks.com/bs/1273/.
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Publication History

• Published on May 3, 2009

Financial Disclosures

• Dr. Tim Yen has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.